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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Overview
Product name
Anti-APPBP1 antibody
See all APPBP1 products (4) ...
Description
Mouse monoclonal to APPBP1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-535 of Human APPBP1
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
-
Western blot - APPBP1 antibody (ab56420)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Tissue specificity
Ubiquitous in fetal tissues. Expressed throughout the adult brain.
Pathway
Protein modification; protein neddylation.
Sequence similarities
Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.
Cellular localization
Cell membrane. Colocalizes with APP in lipid rafts.
Target information above from: UniProt accessionQ13564
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 59 kDa antibody
- A 116A10.1 antibody
- Amyloid beta precursor protein binding protein 1 59kD antibody
see all
Database links
- Entrez Gene: 8883 Human
- Omim: 603385 Human
- SwissProt: Q13564 Human
- Unigene: 460978 Human
Anti-APPBP1 antibody images:
Western blot - APPBP1 antibody (ab56420)
APPBP1 antibody (ab56420) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-APPBP1 antibody (ab56420)
ab56420 has not yet been referenced specifically in any publications.
Publishing research using ab56420? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"