Anti-ARD1A antibody [3G3B9] (ab234076)
Key features and details
- Mouse monoclonal [3G3B9] to ARD1A
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, African green monkey, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-ARD1A antibody [3G3B9]
See all ARD1A primary antibodies -
Description
Mouse monoclonal [3G3B9] to ARD1A -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human ARD1A aa 111-235. Expressed in E. coli.
Database link: P41227 -
Positive control
- Flow Cytometry: SMMC-7721 cells. ICC/IF: HeLa cells. WB: COS-7, HEK-293, HL-60, MCF7, HeLa, NIH/3T3, and C2C12 cell lysates; Recombinant human ARD1A (amino acids 111-235) protein; Human ARD1A (amino acids 111-235)-hIgGFc transfected HEK-293 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3G3B9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/500. Predicted molecular weight: 26 kDa.
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Flow Cyt |
1/200 - 1/400.
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ICC/IF |
1/200 - 1/1000.
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Notes |
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WB
1/100 - 1/500. Predicted molecular weight: 26 kDa. |
Flow Cyt
1/200 - 1/400. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the acetyltransferase family. ARD1 subfamily.
Contains 1 N-acetyltransferase domain. -
Post-translational
modificationsCleaved by caspases during apoptosis. -
Cellular localization
Cytoplasm. Nucleus. According to PubMed:12464182 it is cytoplasmic. According to PubMed:15496142, it is nuclear and cytoplasmic. Also present in the free cytosolic and cytoskeleton-bound polysomes. - Information by UniProt
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Database links
- Entrez Gene: 8260 Human
- Entrez Gene: 56292 Mouse
- Omim: 300013 Human
- SwissProt: P41227 Human
- SwissProt: Q9QY36 Mouse
- Unigene: 433291 Human
- Unigene: 246654 Mouse
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Alternative names
- Alpha N acetyltransferase 1A antibody
- ARD1 antibody
- ARD1 homolog N acetyltransferase (S. cerevisiae) antibody
see all
Images
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All lanes : Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 6 : NIH-3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 7 : C2C12 (mouse myoblast cell line) cell lysate
Predicted band size: 26 kDa -
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARD1A (green) using ab234076 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AlexaFluor®555-Phalloidin.
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Flow Cytometry analysis of SMMC-7721 (human hepatocarcinoma cell line) cells, labeling ARD1A with ab234076 at a 1/200 dilution (green) compared to a negative control (red).
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All lanes : Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Human ARD1A (amino acids 111-235)-hIgGFc transfected HEH-293 cell lysate
Predicted band size: 26 kDa -
Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution + Recombinant human ARD1A (amino acids 111-235) protein
Predicted band size: 26 kDaExpected recombinant MW is 47.2 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab234076 has not yet been referenced specifically in any publications.