Anti-AREB6 antibody (ab81972)

Overview

  • Product nameAnti-AREB6 antibodySee all AREB6 primary antibodies ...
  • Description
    Goat polyclonal to AREB6
  • Specificityab81972 is expected to recognize both reported isoforms (NP_001121600.1; NP_110378.3).
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Hamster, Cow, Xenopus laevis, Cynomolgus Monkey, Orangutan
  • Immunogen

    Synthetic peptide: C-RPQIRQKIENKPLQE, corresponding to internal sequence amino acids 309-323 of human AREB6 according to NP_001121600.1 (or 325-339 of NP_110378.3).

  • Positive control
    • Human and mouse heart lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab81972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
  • Application notesPeptide ELISA: antibody detection limit dilution 1:32,000.
    WB: Use at a concentration of 0.05 - 0.2 µg/ml. Predicted molecular weight: 124 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
    • Tissue specificityColocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas.
    • Involvement in diseaseDefects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
      Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition.
    • Sequence similaritiesBelongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
      Contains 7 C2H2-type zinc fingers.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localizationNucleus.
    • Target information above from: UniProt accession P37275 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • AREB 6 antibody
      • BZP antibody
      • Delta crystallin enhancer binding factor 1 antibody
      • DELTA EF1 antibody
      • FECD6 antibody
      • MGC133261 antibody
      • Negative regulator of IL 2 antibody
      • Negative regulator of IL2 antibody
      • NIL 2 A antibody
      • NIL 2 A zinc finger protein antibody
      • NIL 2A antibody
      • NIL-2-A zinc finger protein antibody
      • NIL2A antibody
      • Posterior polymorphous corneal dystrophy 3 antibody
      • PPCD3 antibody
      • Represses interleukin 2 expression antibody
      • TCF 8 antibody
      • TCF-8 antibody
      • TCF8 antibody
      • Transcription factor 8 (represses interleukin 2 expression) antibody
      • Transcription factor 8 antibody
      • ZEB 1 antibody
      • ZEB antibody
      • ZEB1 antibody
      • ZEB1_HUMAN antibody
      • ZFHEP antibody
      • ZFHX 1A antibody
      • ZFHX1A antibody
      • Zinc finger E box binding homeobox 1 antibody
      • Zinc finger E-box-binding homeobox 1 antibody
      • Zinc finger homeodomain enhancer binding protein antibody
      see all

    Anti-AREB6 antibody images

    • Anti-AREB6 antibody (ab81972) at 0.05 µg/ml + mouse heart lysate in ripa buffer at 35 µg
      developed using the ECL technique

      Predicted band size : 124 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 hour

    References for Anti-AREB6 antibody (ab81972)

    ab81972 has not yet been referenced specifically in any publications.

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    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Embryonic Stem cell)
    Specification Embryonic Stem cell
    Fixative 1:1 methanol:acetone
    Permeabilization Yes - 0.1% triton-x
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
    Username

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    Verified customer

    Submitted Dec 17 2010

    Application Western blot
    Sample Mouse Cell lysate - whole cell (embryonic stem cell)
    Loading amount 200000 cells
    Specification embryonic stem cell
    Gel Running Conditions Reduced Denaturing (10)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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    Abcam user community

    Verified customer

    Submitted Dec 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"