Anti-ZEB1 antibody (ab81972)
Key features and details
- Goat polyclonal to ZEB1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-ZEB1 antibody
See all ZEB1 primary antibodies -
Description
Goat polyclonal to ZEB1 -
Host species
Goat -
Specificity
ab81972 is expected to recognize both reported isoforms (NP_001121600.1; NP_110378.3). -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Chicken, Hamster, Cow, Xenopus laevis, Cynomolgus monkey, Orangutan -
Immunogen
Synthetic peptide corresponding to Human ZEB1 aa 309-323 (internal sequence). According to NP_001121600.1 (or 325-339 of NP_110378.3).
Sequence:C-RPQIRQKIENKPLQE
Database link: NP_001121600.1 -
Positive control
- ICC/IF: U2OS cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.05 - 0.2 µg/ml. Predicted molecular weight: 124 kDa.
A 1 hour primary incubation is recommended for this product. |
ICC/IF | (1) |
Use a concentration of 10 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.05 - 0.2 µg/ml. Predicted molecular weight: 124 kDa. A 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
-
Function
Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. -
Tissue specificity
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. -
Involvement in disease
Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. -
Sequence similarities
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Contains 7 C2H2-type zinc fingers.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 535183 Cow
- Entrez Gene: 21417 Mouse
- SwissProt: Q64318 Mouse
- Unigene: 3929 Mouse
-
Alternative names
- AREB 6 antibody
- AREB6 antibody
- BZP antibody
see all
Images
-
Anti-ZEB1 antibody (ab81972) at 0.05 µg/ml + Mouse heart tissue lysate in ripa buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour -
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
Datasheets and documents
-
SDS download
-
Datasheet download
References (7)
ab81972 has been referenced in 7 publications.
- Huang J et al. LncRNA SNHG15 regulates EGFR-TKI acquired resistance in lung adenocarcinoma through sponging miR-451 to upregulate MDR-1. Cell Death Dis 11:525 (2020). PubMed: 32655137
- Ni J et al. Human trophoblast-derived exosomes attenuate doxorubicin-induced cardiac injury by regulating miR-200b and downstream Zeb1. J Nanobiotechnology 18:171 (2020). PubMed: 33218341
- Liu H et al. MiR-194-5p inhibited metastasis and EMT of nephroblastoma cells through targeting Crk. Kaohsiung J Med Sci 36:265-273 (2020). PubMed: 31889432
- Chen J et al. The anti-inflammation, anti-oxidative and anti-fibrosis properties of swertiamarin in cigarette smoke exposure-induced prostate dysfunction in rats. Aging (Albany NY) 11:10409-10421 (2019). PubMed: 31739286
- Fu D et al. Hsa_circ_0057481 promotes laryngeal cancer proliferation and migration by modulating the miR-200c/ZEB1 axis. Int J Clin Exp Pathol 12:4066-4076 (2019). PubMed: 31933802
- Huang X et al. Adam12 and lnc015192 act as ceRNAs in breast cancer by regulating miR-34a. Oncogene N/A:N/A (2018). PubMed: 30042416
- Liu L et al. ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer. PLoS One 11:e0148774 (2016). WB . PubMed: 26882471