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Our Abpromise guarantee covers the use of ab11038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.|
ab11038 detecting ARF1 in AGS Human gastric carcinoma cells by sandwich ELISA (detection). Samples were blocked with 5% BSA for 1 hour at 23°C and were incubated with the primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. Anti-mouse HRP (ab6728) (1/1000) was used as the secondary antibody.
The Capture antibody was ab436 - a mouse anti-human IgG (20 ug/ml, 4°C overnight)