Overview

  • Product nameAnti-ARF1 antibody [ARFS 3F1]
    See all ARF1 primary antibodies
  • Description
    Mouse monoclonal [ARFS 3F1] to ARF1
  • SpecificityThis antibody is specific to class I ARF.
  • Tested applicationsIHC-P, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    SNQLRNQ

    , corresponding to C terminal amino acids 174-180 of Human ARF1.

Properties

Applications

Our Abpromise guarantee covers the use of ab11038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 22 kDa.

Target

  • FunctionGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
  • Cellular localizationGolgi apparatus. Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP Ribosylation Factor 1 antibody
    • ADP-ribosylation factor 1 antibody
    • ARF 1 antibody
    • ARF1 antibody
    • ARF1_HUMAN antibody
    • DJ133P16.1 antibody
    see all

Anti-ARF1 antibody [ARFS 3F1] images

  • ab11038 detecting ARF1 in AGS Human gastric carcinoma cells by sandwich ELISA (detection). Samples were blocked with 5% BSA for 1 hour at 23°C and were incubated with the primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. Ab6728 (1/1000) was used as the secondary antibody.
    The Capture antibody was ab436 - a mouse anti-human IgG (20 ug/ml, 4°C overnight)

    See Abreview

  • IHC image of ab11038 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11038, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab11038 staining ARF1 in the Panc-1 cell line from Human pancreatic carcinoma by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formalddehyde and blocked with 1% Donkey serum in PBST for 60 minutes at 22°C. Samples were incubated with primary antibody (1/50 in PBST + 1% Donkey serum) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-ARF1 antibody [ARFS 3F1] (ab11038)

This product has been referenced in:
  • Pye DS  et al. Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from impaired endosomal recycling of Rap1 and is associated with a distinctive type of immunological anergy. J Immunol 191:1496-504 (2013). Read more (PubMed: 23804711) »
  • Lim J  et al. The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling. Genes Dev 24:1496-506 (2010). WB ; Human . Read more (PubMed: 20634316) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Panc-1)
Specification Panc-1
Fixative Formaldehyde
Permeabilization No
Blocking step 1% Donkey serum in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Mr. Armen Petrosyan

Verified customer

Submitted Dec 05 2012

Thank you for contacting us.

I am sorry to hear that you are experiencing issues with this. I was wondering if you could help me to understand this problem by providing a few more details.

Do you know the amount of total protein you a...

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Thank you for your enquiry,

I can confirm the ARF1 protein has a molecular weight of around 20 kDa. There is a Western blotAbreview published on the online datasheet, including an image which shows aband at about 20 kDa.

http://ww...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (AGS human gastric carcinoma cells)
Specification AGS human gastric carcinoma cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Type Sandwich (Detection)
Username

Abcam user community

Verified customer

Submitted Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Loading amount 10 µg
Specification AGS Gastric Carcinoma cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 18 2010

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 50 µg
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Apr 05 2007

Thank you for your enquiry. The originator of ab11038 has told me that this antibody is class 1 ARFS specific. If you have any additional questions, please contact us again.

Thank you for your enquiry. All the information that we have regarding ab11038 is located on the online datasheet. Unfortunately, we are not aware of any publications that feature the use of this antibody and do not have any images to provide at this t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"