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Western blot - ARF4 antibody (ab75073)
(enlarge)
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Product Name
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ARF4 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ARF4
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Immunogen
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Synthetic peptide (Human)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Specificity
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ARF4 antibody detects endogenous levels of total ARF4 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
WB: 1/500 - 1/1000. Predicted molecular weight: 21 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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LOVO cell lysate, K562 cell lysate, HT-29 cell lysate
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Cellular localization
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Golgi Apparatus
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Research areas
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Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
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Relevance
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ARF4 is a member of the ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ARF4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ARF4 antibody images, references, products related to ab75073 and other tools.
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ARF4 antibody images:
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 Western blot - ARF4 antibody (ab75073)
All lanes : ARF4 antibody (ab75073) at 1/1000 dilution
Lane 1 : LOVO cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HT-29 cell lysate
Lane 4 : K562 cell lysate with Blocking peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 21 kDa
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Search PubMed (MEDLINE) for references to ARF4
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ARF4 antibody - more information
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Customer reviews (feedback) regarding ARF4 antibody |
Customer FAQs regarding ARF4 antibody |
Protocols for ARF4 antibody |
Price and availability of products related to ARF4 antibody |
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ARF4 antibody - related products:
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ARF4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ARF4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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