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Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Overview
Product name
Anti-ARF4 antibody
Description
Rabbit polyclonal to ARF4
Specificity
ARF4 antibody detects endogenous levels of total ARF4 protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide (Human)
Positive control
LOVO cell lysate, K562 cell lysate, HT-29 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
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Western blot - ARF4 antibody (ab75073)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 21 kDa.
ELISA
ELISA: 1/40000ELISA: 1/40000
Target
Relevance
ARF4 is a member of the ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene.
Cellular localization
Golgi Apparatus
Alternative names
- ADP ribosylation factor 2 antibody
- ADP ribosylation factor 4 antibody
- ARF2 antibody
Database links
- Entrez Gene: 378 Human
- Entrez Gene: 11843 Mouse
- Entrez Gene: 79120 Rat
- Omim: 601177 Human
- SwissProt: P18085 Human
- SwissProt: P61750 Mouse
- SwissProt: P61751 Rat
Anti-ARF4 antibody images:
Western blot - ARF4 antibody (ab75073)
All lanes : Anti-ARF4 antibody (ab75073) at 1/1000 dilution
Lane 1 : LOVO cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HT-29 cell lysate
Lane 4 : K562 cell lysate with Blocking peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 21 kDa
References for Anti-ARF4 antibody (ab75073)
ab75073 has not yet been referenced specifically in any publications.
Publishing research using ab75073? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"