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Overview

  • Product nameARF4 protein (Tagged-His Tag)See all ARF4 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 10-180 of Human ARF4 with an N-terminal His tag; Predicted MWt 20 kDa (UniProt P18085).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 87 µl aqua dest
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTI GFNVETVEYKN ICFTVWDVGGQDRIRPLWKHYFQNTQG LIFVVDSNDRERIQEVADELQKML LVDELRDAVLLLFA NKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQ G TGLYEGLDWLSNELSKR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91665 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ADP ribosylation factor 2ADP ribosylation factor 4ADP-ribosylation factor 4
        ARF2ARF4ARF4_HUMAN
      see all
  • FunctionGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
  • Cellular localizationGolgi apparatus.

    • Target information above from: UniProt accession P18085 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ARF4 protein (Tagged-His Tag) images

    • SDS-PAGE - ARF4 protein (Tagged-His Tag) (ab91665)
      SDS-PAGE - ARF4 protein (Tagged-His Tag) (ab91665)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ARF4 protein (Tagged-His Tag) (ab91665)

    ab91665 has not yet been referenced specifically in any publications.

    Publishing research using ab91665 ? Please let us know so that we can cite the reference in this datasheet

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