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Product Name
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ARF6 antibody [ARFAG]
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See all ARF6 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [ARFAG] to ARF6
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Immunogen
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Synthetic peptide: LTRIRDRNWYVQPSC conjugated to KLH, corresponding to amino acids 141-155 of Human ARF6 BLAST 'LTRIRDRNWYVQPSC' with or 
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Reacts with
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Hu, Rat, Cow, Dog
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Predicted to react
(species key)
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Ms, Chk, Pig, Xl (Due to sequence homology)
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Tested applications
(see key)
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ELISA, ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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MDCK total cell extract
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Cellular localization
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Golgi Apparatus
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
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Relevance
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ARF6 (ADP ribosylation factor 6) is a member of the human ARF gene family. These genes encode small guanine nucleotide binding proteins that stimulate the ADP ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking during cellular differentiation and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. ARF6 is a broadly expressed, differentially regulated GTPase that is present in cytoplasm and on both cell surface and intracellular membranes.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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ARFAG
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Myeloma
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NS1
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 15mM Sodium Azide Constituents: 0.01mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 2.00 - 2.30 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ARF6 antibody [ARFAG] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to ARF6
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ARF6 antibody [ARFAG] - more information
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