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Product Name 

ARF6 antibody [ARFAG]

 

See all ARF6 antibodies (6)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [ARFAG] to ARF6

Immunogen 

Synthetic peptide: LTRIRDRNWYVQPSC conjugated to KLH, corresponding to amino acids 141-155 of Human ARF6
BLAST 'LTRIRDRNWYVQPSC' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu, Rat, Cow, Dog

Predicted to react 
(species key)

Ms, Chk, Pig, Xl
(Due to sequence homology)

Tested applications 
(see key)

ELISA, ICC, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MDCK total cell extract

Cellular localization 

Golgi Apparatus

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation

Relevance 

ARF6 (ADP ribosylation factor 6) is a member of the human ARF gene family. These genes encode small guanine nucleotide binding proteins that stimulate the ADP ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking during cellular differentiation and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. ARF6 is a broadly expressed, differentially regulated GTPase that is present in cytoplasm and on both cell surface and intracellular membranes.

 

Alternative names ARF6 antibody [ARFAG] (ab49931)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

428927    (Chicken)
382    (Human)
11845    (Mouse)
79121    (Rat)
399300    (Xenopus laevis)

Omim

    

600464    (Human)

SwissProt

    

P26990    (Chicken)
P62330    (Human)
P62331    (Mouse)
P62332    (Rat)
P51645    (Xenopus laevis)

Unigene

    

525330    (Human)
6993    (Rat)
963    (Xenopus laevis)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

ARFAG

Myeloma 

NS1

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01mM PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 2.00 - 2.30 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ARF6 antibody [ARFAG] is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to ARF6

 

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Other ARF6 Antibodies

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