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Western blot - ARF6 antibody (ab77581)
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Immunocytochemistry/ Immunofluorescence - ARF6 antibody (ab77581)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARF6 antibody (ab77581)
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Product Name
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ARF6 antibody
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See all ARF6 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ARF6
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus ARF6.
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Chk, Cow, Pig, Xl
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following whole cell lysates:
HeLa whole cell, HEK293 whole cell, HepG2 whole cell, Human Liver Tissue
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Cellular localization
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Golgi Apparatus
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
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Relevance
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ARF6 (ADP ribosylation factor 6) is a member of the human ARF gene family. These genes encode small guanine nucleotide binding proteins that stimulate the ADP ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking during cellular differentiation and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. ARF6 is a broadly expressed, differentially regulated GTPase that is present in cytoplasm and on both cell surface and intracellular membranes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ARF6 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ARF6 antibody images, references, products related to ab77581 and other tools.
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ARF6 antibody images:
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 Western blot - ARF6 antibody (ab77581)
All lanes : ARF6 antibody (ab77581) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
We hypothesize that the 18 kDa band represents the mature form of ARF6.
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Immunocytochemistry/ Immunofluorescence - ARF6 antibody (ab77581)
ICC/IF image of ab77581 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77581, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 1µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARF6 antibody (ab77581)
IHC image of ARF6 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77581, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - ARF6 antibody (ab77581)
All lanes : ARF6 antibody (ab77581) at 1 µg/ml
Lane 1 : Lysates prepared from Mouse liver tissue.
Lane 2 : Lysates prepared from Mouse lung tissue.
Lane 3 : Lysates prepared from Rat liver tissue.
Lane 4 : Lysates prepared from Rat lung tissue.
Lysates/proteins at 20 µg per lane.
Secondary
HRP-conjugated Pig polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes This image is a courtesy of Antibody Solutions Ltd
See Abreview
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Search PubMed (MEDLINE) for references to ARF6
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ARF6 antibody - more information
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Customer reviews (feedback) regarding ARF6 antibody |
Customer FAQs regarding ARF6 antibody |
Protocols for ARF6 antibody |
Price and availability of products related to ARF6 antibody |
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ARF6 antibody - related products:
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ARF6 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ARF6 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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