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Products:Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Overview
Product name
Anti-ARF6 antibody
See all ARF6 products (6) ...
Description
Rabbit polyclonal to ARF6
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Dog, Pig, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Zebrafish
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 110-159 (REMRDAIILI FANKQDLPDA MKPHEIQEKL GLTRIRDRNW YVQPSCATSG) of human ARF6 (NP_001654).
REMRDAIILI FANKQDLPDA MKPHEIQEKL GLTRIRDRNW YVQPSCATSG
Positive control
MCF7 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified by peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
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Western blot - ARF6 antibody (ab81650)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ARF6 (ADP ribosylation factor 6) is a member of the human ARF gene family. These genes encode small guanine nucleotide binding proteins that stimulate the ADP ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking during cellular differentiation and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. ARF6 is a broadly expressed, differentially regulated GTPase that is present in cytoplasm and on both cell surface and intracellular membranes.
Cellular localization
Golgi apparatus. Cell membrane; Lipid-anchor. Endosome membrane; Lipid-anchor.
Alternative names
- antibody
- Small GTPase antibody
- ADP ribosylation factor 6 antibody
see all
Database links
- Entrez Gene: 382 Human
- Entrez Gene: 11845 Mouse
- Entrez Gene: 79121 Rat
- Omim: 600464 Human
- SwissProt: P62330 Human
- SwissProt: P62331 Mouse
- SwissProt: P62332 Rat
- Unigene: 525330 Human
see all
Anti-ARF6 antibody images:
Western blot - ARF6 antibody (ab81650)
Anti-ARF6 antibody (ab81650) at 1 µg/ml + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 20 kDa
Observed band size : <22 kDa (why is the actual band size different from the predicted?)
References for Anti-ARF6 antibody (ab81650)
ab81650 has not yet been referenced specifically in any publications.
Publishing research using ab81650? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"