Feedback Form

Western blot - ARF6 antibody (ab81650)
Western blot - ARF6 antibody (ab81650)
(enlarge)


Product Name 

ARF6 antibody

 

See all ARF6 antibodies (6)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ARF6

Immunogen 

Synthetic peptide corresponding to a region within N terminal amino acids 110-159 (REMRDAIILI FANKQDLPDA MKPHEIQEKL GLTRIRDRNW YVQPSCATSG) of human ARF6 (NP_001654).
BLAST 'REMRDAIILI FANKQDLPDA MKPHEIQEKL GLTRIRDRNW YVQPSCATSG ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat, Dm

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MCF7 cell lysate

Cellular localization 

Golgi Apparatus

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation

Relevance 

ARF6 (ADP ribosylation factor 6) is a member of the human ARF gene family. These genes encode small guanine nucleotide binding proteins that stimulate the ADP ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking during cellular differentiation and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. ARF6 is a broadly expressed, differentially regulated GTPase that is present in cytoplasm and on both cell surface and intracellular membranes.

 

Alternative names ARF6 antibody (ab81650)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

382    (Human)
11845    (Mouse)
79121    (Rat)

Omim

    

600464    (Human)

SwissProt

    

P62330    (Human)
P62331    (Mouse)
P62332    (Rat)

Unigene

    

525330    (Human)
6993    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Purification notes 

Purified by peptide affinity chromatography method.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ARF6 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ARF6 antibody images, references, products related to ab81650 and other tools.

 

ARF6 antibody images:

Product image  Western blot - ARF6 antibody (ab81650)

Western blot - ARF6 antibody (ab81650)

ARF6 antibody (ab81650) at 1 µg/ml + MCF7 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 20 kDa
Observed band size : <22 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to ARF6

 

ARF6 antibody - more information

Customer reviews (feedback) regarding ARF6 antibody

Customer FAQs regarding ARF6 antibody

Protocols for ARF6 antibody

Price and availability of products related to ARF6 antibody

 

ARF6 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for ARF6 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other ARF6 Antibodies

See all ARF6 antibodies (6)...

 

ARF6 antibody - other tools:

Contact Abcam with a Technical Enquiry about ARF6 antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.