Anti-ARH antibody - Carboxyterminal end (ab63155)

Overview

• Product nameAnti-ARH antibody - Carboxyterminal endSee all ARH primary antibodies ...
• Description
  Rabbit polyclonal to ARH - Carboxyterminal end
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide (Human) conjugated to KLH to the C terminal region of human ARH.

• Positive controlNCI-H460 cell line lysates and human hepatocarcinoma tissue.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Cholesterol Metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipoprotein metabolism

  • Metabolism
  • Types of disease
  • Heart disease

  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Cardiovascular
  • Atherosclerosis
  • Lipoprotein metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cholesterol Metabolism

Applications

Target

• FunctionAdapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface.
• Tissue specificityExpressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes.
• Involvement in diseaseDefects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH) [MIM:603813]. ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. ARH has the clinical features of familial hypercholesterolemia (FH) [MIM:143890] homozygotes, including severely elevated plasma LDL cholesterol, tuberous and tendon xanthomata, and premature atherosclerosis. LDL receptor (LDLR) activity measured in skin fibroblasts is normal, as the LDL binding ability.
• Sequence similaritiesContains 1 PID domain.
• DomainThe [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction the AP-2 complex subunit AP2B1.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q5SW96 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 26119 Human
  • Omim: 605747 Human
  • SwissProt: Q5SW96 Human
  • Unigene: 590911 Human

• Alternative names
  ARH antibodyARH GENE antibodyARH_HUMAN antibody

  ARH1 antibodyARH2 antibodyAutosomal recessive hypercholesterolemia protein antibodyDKFZp586D0624 antibodyFHCB1 antibodyFHCB2 antibodyLDL receptor adaptor protein antibodyLdlrap1 antibodyLow density lipoprotein receptor adapter protein 1 antibodyMGC34705 antibodyOTTHUMP00000008526 antibody

  see all

Anti-ARH antibody - Carboxyterminal end images

• 

  Western blot - ARH antibody - Carboxyterminal end (ab63155)
  Anti-ARH antibody - Carboxyterminal end (ab63155) at 1/60 dilution + NCI-H460 cell line lysates at 35 µg
  
  Predicted band size : 34 kDa
  Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARH antibody - Carboxyterminal end (ab63155)
  Immunohistochemical analysis of ARH expression in formalin fixed, paraffin embedded human hepatocarcinoma tissue using 1/50 ab63155, followed by peroxidase-conjugated secondary antibody step and DAB staining.