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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - ARHGAP20 antibody (ab68918)
Anti-ARHGAP20 antibody (ab68918) at 1/500 dilution + rat brain lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 132 kDa
Observed band size : 132 kDa
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Western blot - ARHGAP20 antibody (ab68918)
All lanes : Anti-ARHGAP20 antibody (ab68918) at 1/500 dilution
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 132 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
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