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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-ARHGAP20 antibody
See all ARHGAP20 products (2) ...
Description
Mouse polyclonal to ARHGAP20
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
ARHGAP20 (NP_065860, 1 a.a. ~ 1191 a.a) human protein.
Positive control
Rat brain tissue lysate, ARHGAP20 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 150 kDa (predicted molecular weight: 132 kDa).
Target
Function
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
Tissue specificity
Expressed predominantly in the brain. Lower expression is found in lymph nodes.
Involvement in disease
Note=A chromosomal aberration involving ARHGAP20 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(X;11)(q21;q23) with BRWD3 does not result in fusion transcripts but disrupts both genes.
Sequence similarities
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 Rho-GAP domain.
Developmental stage
Low expression is found in fetal liver.
Target information above from: UniProt accessionQ9P2F6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Arhgap20 antibody
- KIAA1391 antibody
- RARHOGAP antibody
see all
Database links
- Entrez Gene: 57569 Human
- Omim: 609568 Human
- SwissProt: Q9P2F6 Human
- Unigene: 6136 Human
Anti-ARHGAP20 antibody images:
Western blot - ARHGAP20 antibody (ab68918)
Anti-ARHGAP20 antibody (ab68918) at 1/500 dilution + rat brain lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 132 kDa
Observed band size : 132 kDa
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Western blot - ARHGAP20 antibody (ab68918)
All lanes : Anti-ARHGAP20 antibody (ab68918) at 1/500 dilution
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 132 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
References for Anti-ARHGAP20 antibody (ab68918)
ab68918 has not yet been referenced specifically in any publications.
Publishing research using ab68918? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"