Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Overview
Product name
Anti-ARIH2 antibody
See all ARIH2 products (5) ...
Description
Goat polyclonal to ARIH2
Tested applications
WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow, Dog, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide: EQRRRTLLKDFHDT, corresponding to C terminal amino acids 480-493 of Human ARIH2.
EQRRRTLLKD FHDT
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 3 µg/ml.(Customer's experiments gave a band at approx 60kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8kDa according to NP_006312.1).)
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Target
Function
E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
Tissue specificity
Widely expressed with higher expression in granulocytes.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the RBR family. Ariadne subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
Domain
RING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.
Post-translational
modifications
Ubiquitinated. Ubiquitination promotes proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Cytoplasm.
Target information above from: UniProt accessionO95376
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HT005 antibody
- Ari-2 antibody
- ARI2 antibody
see all
Database links
- Entrez Gene: 10425 Human
- Entrez Gene: 23807 Mouse
- Entrez Gene: 406417 Zebrafish
- Omim: 605615 Human
- SwissProt: O95376 Human
- SwissProt: Q9Z1K6 Mouse
- Unigene: 241558 Human
- Unigene: 633601 Human
see all
References for Anti-ARIH2 antibody (ab17014)
ab17014 has not yet been referenced specifically in any publications.
Publishing research using ab17014? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"