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All lanes : Anti-ARIH2 antibody (ab52678) at 1/1000 dilution
Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (recombinant fusion protein containing a His tag).
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution
Predicted band size : 59 kDa
Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.
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