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Overview
Product name
Anti-ARIH2 antibody
See all ARIH2 products (5) ...
Description
Mouse polyclonal to ARIH2
Tested applications
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Vector coding for a partial recombinant fusion protein, (His tagged), corresponding to amino acids 11-41 of Fruit fly (Drosophila melanogaster) ARIH2. Target sequence used to make the antibody: SDNDGEYDDDYDYYNTGEDCDVERLDPKRA.
SDNDGEYDDD YDYYNTGEDC DVERLDPKRA
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Western blot - ARIH2 antibody (ab52678)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 59 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
Tissue specificity
Widely expressed with higher expression in granulocytes.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the RBR family. Ariadne subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
Domain
RING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.
Post-translational
modifications
Ubiquitinated. Ubiquitination promotes proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Cytoplasm.
Target information above from: UniProt accessionO95376
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HT005 antibody
- Ari-2 antibody
- ARI2 antibody
see all
Database links
- SwissProt: O76924 Fruit fly (Drosophila melanogaster)
Anti-ARIH2 antibody images:
Western blot - ARIH2 antibody (ab52678)
All lanes : Anti-ARIH2 antibody (ab52678) at 1/1000 dilution
Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (recombinant fusion protein containing a His tag).
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution
Predicted band size : 59 kDa
Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.
References for Anti-ARIH2 antibody (ab52678)
ab52678 has not yet been referenced specifically in any publications.
Publishing research using ab52678? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"