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Overview

  • Product nameAnti-ARNT+ARNT2 antibody
  • Description
    Rabbit polyclonal to ARNT+ARNT2
  • Tested applicationsELISA, IHC-P, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    KLH conjugated synthetic peptide: KTGTVKKEGQQSSMRCA, corresponding to N terminal amino acids 214-228 of Human ARNT/ARNT2 containing a predicted sumoylation site.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlK562 cell line lysate and human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
IHC-P
WB
  • Application notesELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/250 - 1/500. Detects a band of approximately 78 kDa and 86kDa
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceThe aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with leukemia. Sumoylation of ARNT modulates the ability of ARNT to interact with cooperative molecules such as PML, thereby regulating the transcriptional role of ARNT. This exemplifies a crucial role of protein sumoylation in modulating protein-protein interactions.
    • Cellular localizationNuclear
    • Database links
      see all
    • Alternative names
        ARNT protein 2 antibodyARNT2 antibodyAryl hydrocarbon receptor nuclear translocator antibody
        Aryl hydrocarbon receptor nuclear translocator 2 antibodyBHLHE2 antibodyDioxin receptor, nuclear translocator antibodyHIF1B antibodyHIF1BETA antibodyTANGO antibody
      see all

    Anti-ARNT+ARNT2 antibody images

    • Western blot - ARNT/ARNT2 antibody (ab71719)
      Western blot - ARNT/ARNT2 antibody (ab71719)
      Anti-ARNT+ARNT2 antibody (ab71719) at 1/250 dilution + K562 cell line lysate at 12.5 µg

      Predicted band size : 78 kDa
      Observed band size : 78 kDa
      Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARNT2 antibody (ab71719)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARNT2 antibody (ab71719)
      ab71719, at 1/50 dilution, staining ARNT2 in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. ab71719 was peroxidase conjugated to the secondary antibody, followed by DAB staining

    References for Anti-ARNT+ARNT2 antibody (ab71719)

    ab71719 has not yet been referenced specifically in any publications.

    Publishing research using ab71719 ? Please let us know so that we can cite the reference in this datasheet

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