Overview
- Product nameAnti-ARP2/3 subunit 1B antibodySee all ARP2/3 subunit 1B primary antibodies ...
- DescriptionGoat polyclonal to ARP2/3 subunit 1B
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Zebrafish - Immunogen
Synthetic peptide (AYHSFLVEPISCH) from N terminus amino acids 2-14 of ARP2/3 subunit 1B (Human)
- Positive controlHuman Thymus tissue, Human liver lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab115217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 41 kDa. |
| IHC-P | IHC-P: Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- FunctionFunctions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
- Sequence similaritiesBelongs to the WD repeat ARPC1 family.
Contains 6 WD repeats. - Cellular localizationCytoplasm > cytoskeleton.
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Database links
- Entrez Gene: 416490 Chicken
- Entrez Gene: 326600 Cow
- Entrez Gene: 10095 Human
- Entrez Gene: 10095 Human
- Entrez Gene: 11867 Mouse
- Entrez Gene: 100174964 Pig
- Entrez Gene: 54227 Rat
- Omim: 604223 Human
- SwissProt: Q58CQ2 Cow
- SwissProt: O15143 Human
- SwissProt: O15143 Human
- SwissProt: Q9WV32 Mouse
- SwissProt: O88656 Rat
- Unigene: 23192 Cow
- Unigene: 489284 Human
- Unigene: 30010 Mouse
- Unigene: 2090 Rat
see all
Target information above from: UniProt accession
O15143
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Actin related protein 2/3 complex subunit 1B 41kDa antibodyActin related protein 2/3 complex subunit 1B antibodyActin-related protein 2/3 complex subunit 1B antibody
- ARC1B_HUMAN antibodyARC41 antibodyArp2/3 complex 41 kDa subunit antibodyARP2/3 protein complex subunit p41 antibodyArpc1b antibodyp40 ARC antibodyp41 ARC antibodyp41-Arc antibody
see all
Anti-ARP2/3 subunit 1B antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARP2/3 subunit 1B antibody (ab115217)Human thymus (formalin fixed, paraffin embedded) stained with ab115217 at 2.5 ug/ml followed by biotinylated anti Goat IgG secondary antibody, alkaline phosphatase streptavidin and chromogen. -
Western blot - Anti-ARP2/3 subunit 1B antibody (ab115217)Anti-ARP2/3 subunit 1B antibody (ab115217) at 2 µg/ml + Human Liver lysate in RIPA buffer at 35 µg
Predicted band size : 41 kDa
References for Anti-ARP2/3 subunit 1B antibody (ab115217)
ab115217 has not yet been referenced specifically in any publications.
