Anti-ARPC1B antibody (ab99314)
Key features and details
- Rabbit polyclonal to ARPC1B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ARPC1B antibody
See all ARPC1B primary antibodies -
Description
Rabbit polyclonal to ARPC1B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide corresponding to Human ARPC1B aa 272-332.
Database link: NP_005711.1 -
Positive control
- HeLa, 293T and NIH3T3 cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab99314 was affinity purified using an epitope specific to ARPC1B immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 41 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. -
Sequence similarities
Belongs to the WD repeat ARPC1 family.
Contains 6 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 416490 Chicken
- Entrez Gene: 326600 Cow
- Entrez Gene: 10095 Human
- Entrez Gene: 11867 Mouse
- Entrez Gene: 100174964 Pig
- Omim: 604223 Human
- SwissProt: Q58CQ2 Cow
- SwissProt: O15143 Human
see all -
Alternative names
- Actin related protein 2/3 complex subunit 1B 41kDa antibody
- Actin related protein 2/3 complex subunit 1B antibody
- Actin-related protein 2/3 complex subunit 1B antibody
see all
Images
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ab99314 at 1 µg/ml detecting ARPC1B in HeLa whole cell lysate by WB following IP.
Lane 1: ab99314 at 3µg/mg of lysate.
Lane 2: IP with an antibody which recognizes an downstream epitope of ARPC1B.
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds. -
All lanes : Anti-ARPC1B antibody (ab99314) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : NIH3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab99314 has been referenced in 3 publications.
- Bertier L et al. Nanobodies targeting cortactin proline rich, helical and actin binding regions downregulate invadopodium formation and matrix degradation in SCC-61 cancer cells. Biomed Pharmacother 102:230-241 (2018). PubMed: 29567535
- Bertier L et al. Inhibitory cortactin nanobodies delineate the role of NTA- and SH3-domain-specific functions during invadopodium formation and cancer cell invasion. FASEB J 31:2460-2476 (2017). PubMed: 28235780
- Chorev DS et al. Regulation of focal adhesion formation by a vinculin-Arp2/3 hybrid complex. Nat Commun 5:3758 (2014). PubMed: 24781749