ARP4 (Angiopoietin-Like 4) Human ELISA Kit (ab99974)
Overview
- Product nameARP4 (Angiopoietin-Like 4) Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 20 pg/ml
- Range27.4 pg/ml - 20000 pg/ml
- Recovery
100.4 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 117.8 67% - 137% Serum 95.38 85% - 104% Plasma 88.04 74% - 103% - Species reactivityReacts with: Human
- Product overview
Abcam's ARP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ARP4 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ARP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and ARP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ARP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ARP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Notes
Optimisation may be required with urine samples.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml ARP4 Microplate (12 strips x 8 wells) coated with anti-Human ARP4 1 unit Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody ARP4: biotinylated anti-Human ARP4 2 vials HRP-Streptavidin Concentrate 280x concentrated HRP-conjugated streptavidin 1 x 200µl Human ARP4 Standard (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml -
Research Areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
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Alternative names
- 425O18 1Angiopoietin like 4Angiopoietin related protein 4
- ANGPT L2 ANGPT L4ANGPTL2ANGPTL4 Fasting induced adipose factor FIAFFibrinogen/angiopoietin related proteinHepatic fibrinogen/angiopoietin related protein HFARPMajor histocompatibility complex region NG27NL2Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein PGARpp1158 PPARG angiopoietin related protein PSEC0166 Secreted protein Bk89Weakly similar to angiopoietin 1
see all
- SwissProt: Q9BY76 Human
Applications
Our Abpromise guarantee covers the use of ab99974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
ARP4 (Angiopoietin-Like 4) Human ELISA Kit images
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Typical Standard CurveRepresentative standard curve using ab99974.
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Typical Standard CurveRepresentative standard curve using ab99974.
Protocols
References for ARP4 (Angiopoietin-Like 4) Human ELISA Kit (ab99974)
ab99974 has not yet been referenced specifically in any publications.
