Anti-ARRDC4 antibody (ab74265)
Key features and details
- Rabbit polyclonal to ARRDC4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARRDC4 antibody -
Description
Rabbit polyclonal to ARRDC4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide derived from an internal region of human ARRDC4.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa). |
Target
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Sequence similarities
Belongs to the arrestin family. - Information by UniProt
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Database links
- Entrez Gene: 91947 Human
- Entrez Gene: 66412 Mouse
- Entrez Gene: 293019 Rat
- SwissProt: Q8NCT1 Human
- SwissProt: Q7TP90 Rat
- Unigene: 6093 Human
- Unigene: 14864 Rat
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Alternative names
- ARRD4_HUMAN antibody
- ARRDC 4 antibody
- Arrdc4 antibody
see all
Images
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All lanes : Anti-ARRDC4 antibody (ab74265) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab74265 has been referenced in 1 publication.
- Shea FF et al. Mammalian alpha arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with beta arrestins. PLoS One 7:e50557 (2012). ICC/IF ; Human . PubMed: 23236378