Recombinant Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7220(2)] to ART3 - C-terminal
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ART3 antibody [EPR7220(2)] - C-terminal
See all ART3 primary antibodies -
Description
Rabbit monoclonal [EPR7220(2)] to ART3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human testis tissue; Human testis lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7220(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 41, 44 kDa (predicted molecular weight: 44 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/20.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 41, 44 kDa (predicted molecular weight: 44 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/20. |
Target
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Tissue specificity
Testis specific. -
Sequence similarities
Belongs to the Arg-specific ADP-ribosyltransferase family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 419 Human
- Omim: 603086 Human
- SwissProt: Q13508 Human
- Unigene: 24976 Human
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Alternative names
- ADP ribosyltransferase 3 antibody
- ART 3 antibody
- Art3 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ART3 using ab192870 at 1/500 dilution (0.6 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870) at 1/10000 dilution + Human testis tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 41 kDa (possible mature (processed) protein) -
Immunoprecipitation analysis of Human testis lysate labeling ART3 using ab192870 at 1/20 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibody
Note: The band observed at 41 kDa may be due to the removal of a 27 amino acid propeptide (mature form).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab192870 has been referenced in 1 publication.
- Liu D et al. c-MYC-induced long noncoding RNA MEG3 aggravates kidney ischemia-reperfusion injury through activating mitophagy by upregulation of RTKN to trigger the Wnt/ß-catenin pathway. Cell Death Dis 12:191 (2021). PubMed: 33602903