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Western blot - ART4 antibody (ab83342)
Western blot - ART4 antibody (ab83342)
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Product Name 

ART4 antibody

 

See all ART4 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ART4

Immunogen 

Synthetic peptide corresponding to a region within internal sequence amino acids 179-228 (TLCYEVHYRT KDVHFNAYTG ATIRFGQFLS TSLLKEEAQE FGNQTLFTIF) of Human ART4 (NP_066549).

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay 1/312500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human Fetal heart lysate.

Cellular localization 

Cell membrane; Lipidbanchor, GPI anchor.

Research areas 

Cardiovascular >> Blood >> Blood Cell Antigens >> RBC Antigens >> Blood Group Antigens

Relevance 

ART4 is a member of the ADP ribosyltransferase gene family. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known.

 

ART4 antibody (ab83342)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

420    (Human)

Omim

    

110600    (Human)

SwissProt

    

Q93070    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Lyophilised
Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml.

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ART4 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ART4 antibody images, references, products related to ab83342 and other tools.

 

ART4 antibody images:

Product image  Western blot - ART4 antibody (ab83342)

Western blot - ART4 antibody (ab83342)

ART4 antibody (ab83342) at 1/1 dilution + fetal heart lysate at 10 µg/ml

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 36 kDa
Observed band size : 36 kDa


Gel concentration 12%

 
 

Search PubMed (MEDLINE) for references to ART4

 

ART4 antibody - more information

Customer reviews (feedback) regarding ART4 antibody

Customer FAQs regarding ART4 antibody

Protocols for ART4 antibody

Price and availability of products related to ART4 antibody

 

ART4 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for ART4 antibody

ab29424

  

Heart (Human) Membrane Lysate - fetal normal tissue

 

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ab6564

  

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Other ART4 Antibodies

See all ART4 antibodies (2)...

 

ART4 antibody - other tools:

Contact Abcam with a Technical Enquiry about ART4 antibody

 

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