Overview

  • Product nameAnti-ART4 antibody
    See all ART4 primary antibodies
  • Description
    Rabbit polyclonal to ART4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Pig, Chimpanzee, African Green Monkey
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 179-228 (TLCYEVHYRT KDVHFNAYTG ATIRFGQFLS TSLLKEEAQE FGNQTLFTIF) of Human ART4 (NP_066549).

  • Positive control
    • Human Fetal heart lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

Target

  • RelevanceART4 is a member of the ADP ribosyltransferase gene family. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known.
  • Cellular localizationCell membrane; Lipidbanchor, GPI anchor.
  • Database links
  • Alternative names
    • ADP ribosyltransferase 4 (DO blood group) antibody
    • ADP ribosyltransferase 4 (Dombrock blood group) antibody
    • ADP ribosyltransferase 4 antibody
    • ART 4 antibody
    • CD297 antibody
    • CD297 antigen antibody
    • DO antibody
    • DOK1 antibody
    • Dombrock blood group carrier molecule antibody
    • Ecto ADP ribosyltransferase 4 antibody
    • Mono ADP ribosyltransferase 4 antibody
    • Mono(ADP ribosyl)transferase 4 antibody
    • NAD(P)(+) arginine ADP ribosyltransferase 4 antibody
    see all

Anti-ART4 antibody images

  • Anti-ART4 antibody (ab83342) at 1/1 dilution + fetal heart lysate at 10 µg/ml

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa

References for Anti-ART4 antibody (ab83342)

ab83342 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization Yes - 0.2% Triton X-100
Specification Heart
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Oct 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"