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Products:Cardiovascular >> Blood >> Blood Cell Antigens >> RBC Antigens >> Blood Group Antigens
Overview
Product name
Anti-ART4 antibody
See all ART4 products (2) ...
Description
Rabbit polyclonal to ART4
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 179-228 (TLCYEVHYRT KDVHFNAYTG ATIRFGQFLS TSLLKEEAQE FGNQTLFTIF) of Human ART4 (NP_066549).
Positive control
Human Fetal heart lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Blood Cell Antigens >> RBC Antigens >> Blood Group Antigens
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Western blot - ART4 antibody (ab83342)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay 1/312500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ART4 is a member of the ADP ribosyltransferase gene family. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known.
Cellular localization
Cell membrane; Lipidbanchor, GPI anchor.
Alternative names
- ADP ribosyltransferase 4 (DO blood group) antibody
- ADP ribosyltransferase 4 (Dombrock blood group) antibody
- ADP ribosyltransferase 4 antibody
see all
Database links
- Entrez Gene: 420 Human
- Omim: 110600 Human
- SwissProt: Q93070 Human
Anti-ART4 antibody images:
Western blot - ART4 antibody (ab83342)
Anti-ART4 antibody (ab83342) at 1/1 dilution + fetal heart lysate at 10 µg/ml
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 36 kDa
Observed band size : 36 kDa
Gel concentration 12%
References for Anti-ART4 antibody (ab83342)
ab83342 has not yet been referenced specifically in any publications.
Publishing research using ab83342? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"