Anti-ARTS1 antibody - C-terminal (ab181390)

Overview

  • Product name
    Anti-ARTS1 antibody - C-terminal
    See all ARTS1 primary antibodies
  • Description
    Rabbit polyclonal to ARTS1 - C-terminal
  • Host species
    Rabbit
  • Specificity
    At least two isoforms of ARTS1 are known to exist; ab181390 will detect both isoforms. ab181390 is predicted to not cross-react with ERAP2.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ARTS1 (C terminal). The exact sequence is proprietary. 19 amino acids.
    Database link: Q9NZ08

  • Positive control
    • SK-N-SH cell lysate. Mouse brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab181390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 107 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the peptidase M1 family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A LAP antibody
    • A-LAP antibody
    • Adipocyte derived leucine aminopeptidase antibody
    • Adipocyte-derived leucine aminopeptidase antibody
    • ALAP antibody
    • Aminopeptidase PILS antibody
    • Aminopeptidase regulator of TNFR1 shedding antibody
    • APPILS antibody
    • ARTS 1 antibody
    • ARTS-1 antibody
    • Arts1 antibody
    • Endoplasmic reticulum aminopeptidase 1 antibody
    • Endoplasmic reticulum aminopeptidase associated with antigen processing antibody
    • ERAAP antibody
    • ERAAP1 antibody
    • ERAP 1 antibody
    • Erap1 antibody
    • ERAP1_HUMAN antibody
    • KIAA0525 antibody
    • kiaa0525 protein antibody
    • PILS AP antibody
    • PILS-AP antibody
    • PILSA antibody
    • PILSAP antibody
    • Puromycin insensitive leucyl specific aminopeptidase antibody
    • Puromycin-insensitive leucyl-specific aminopeptidase antibody
    • Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator antibody
    • type 1 tumor necrosis factor receptors shedding aminopeptidase regulator antibody
    • VEGF-induced aminopeptidase antibody
    see all

Images

  • Lane 1 : Anti-ARTS1 antibody - C-terminal (ab181390) at 1 µg/ml
    Lane 2 : Anti-ARTS1 antibody - C-terminal (ab181390) at 2 µg/ml

    All lanes : SK-N-SH whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 107 kDa
    Observed band size: 105 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of mouse brain tissue, staining ARTS1 with ab181390 at 5 µg/ml.

  • Immunofluorescence of mouse brain tissue, staining ARTS1 with ab181390 at 20 μg/mL.

References

ab181390 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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