Overview

  • Product nameAnti-ASAH1 antibodySee all ASAH1 primary antibodies ...
  • Description
    Mouse monoclonal to ASAH1
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: PPWTEDCRKS TYPPSGPTYR GAVPWYTINL DLPPYKRWHE LMLDKAPMLK VIVNSLKNMI NTFVPSGKVM QVVDEKLPGL LGNFPGPFEE EMKGIAAVTD , corresponding to amino acids 25-125 of Human ASAH1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • IsotypeIgG3
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab54822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
    • Tissue specificityBroadly expressed with highest expression in heart.
    • Involvement in diseaseDefects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age.
    • Sequence similaritiesBelongs to the acid ceramidase family.
    • Cellular localizationLysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • AC antibody
      • ACDase antibody
      • Acid CDase antibody
      • Acid ceramidase antibody
      • Acid ceramidase precursor antibody
      • Acid ceramidase subunit beta antibody
      • Acylsphingosine deacylase antibody
      • ASAH 1 antibody
      • ASAH antibody
      • ASAH1 antibody
      • ASAH1_HUMAN antibody
      • FLJ21558 antibody
      • FLJ22079 antibody
      • N acylsphingosine amidohydrolase (acid ceramidase) 1 antibody
      • N acylsphingosine amidohydrolase 1 antibody
      • N acylsphingosine amidohydrolase antibody
      • N-acylsphingosine amidohydrolase antibody
      • N-acylsphingosine deacylase antibody
      • PHP antibody
      • PHP32 antibody
      • Putative 32 kDa heart protein antibody
      • SMAPME antibody
      see all

    Anti-ASAH1 antibody images

    • ASAH1 antibody (ab54822) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart.
    • Western blot against tagged recombinant protein immunogen using ab54822 ASAH1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-ASAH1 antibody (ab54822)

    ab54822 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (PPC1 Cells (Prostate cancer line))
    Loading amount 35 µg
    Specification PPC1 Cells (Prostate cancer line)
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 27 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"