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Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • IsotypeIgG3
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab54822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionHydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
    • Tissue specificityBroadly expressed with highest expression in heart.
    • Involvement in diseaseDefects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age.
    • Sequence similaritiesBelongs to the acid ceramidase family.
    • Cellular localizationLysosome.

      • Target information above from: UniProt accession Q13510 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          AC antibodyACDase antibodyAcid CDase antibody
          Acid ceramidase antibodyAcid ceramidase precursor antibodyAcid ceramidase subunit beta antibodyAcylsphingosine deacylase antibodyASAH 1 antibodyASAH antibodyASAH1 antibodyASAH1_HUMAN antibodyFLJ21558 antibodyFLJ22079 antibodyN acylsphingosine amidohydrolase (acid ceramidase) 1 antibodyN acylsphingosine amidohydrolase 1 antibodyN acylsphingosine amidohydrolase antibodyN-acylsphingosine amidohydrolase antibodyPHP antibodyPHP32 antibodyPutative 32 kDa heart protein antibody
        see all

      Anti-ASAH1 antibody images

      • IHC-P - ASAH1 antibody (ab54822)
        IHC-P - ASAH1 antibody (ab54822)
        ASAH1 antibody (ab54822) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart.
      • Western blot - ASAH1 antibody (ab54822)
        Western blot - ASAH1 antibody (ab54822)
        Western blot against tagged recombinant protein immunogen using ab54822 ASAH1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

      References for Anti-ASAH1 antibody (ab54822)

      ab54822 has not yet been referenced specifically in any publications.

      Publishing research using ab54822 ? Please let us know so that we can cite the reference in this datasheet

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      Western blot abreview for Anti-ASAH1 antibody

       Good
      Abreviews
      Application Western blot
      Sample Human Cell lysate - whole cell (PPC1 Cells (Prostate cancer line))
      Loading amount 35 µg
      Specification PPC1 Cells (Prostate cancer line)
      Gel Running Conditions Reduced Denaturing
      Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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      Submitted Mar 27 2008

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"