The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
FunctionMay be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificityHighest level of expression in skeletal muscle. Also expressed in heart, brain, placenta, liver, kidney and pancreas.
PathwayProtein modification; protein ubiquitination.