Anti-ASB8 antibody (ab67895)

Overview

• Product nameAnti-ASB8 antibodySee all ASB8 primary antibodies ...
• Description
  Mouse polyclonal to ASB8
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length protein, corresponding to amino acids 1-288 of Human ASB8

• Positive controlTransfected 293T cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Whole serum
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E2s and E3s
  • SCF Complex E3 Ligase

Applications

Target

• FunctionMay be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
• Tissue specificityHighest level of expression in skeletal muscle. Also expressed in heart, brain, placenta, liver, kidney and pancreas.
• PathwayProtein modification; protein ubiquitination.
• Sequence similaritiesContains 4 ANK repeats.
  Contains 1 SOCS box domain.
• DomainThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q9H765 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 140461 Human
  • Omim: 615053 Human
  • SwissProt: Q9H765 Human
  • Unigene: 432699 Human

• Alternative names
  Ankyrin repeat and SOCS box containing 8 antibodyAnkyrin repeat and SOCS box protein 8 antibodyASB 8 antibody

  ASB-8 antibodyASB8 antibodyASB8_HUMAN antibodyFLJ21255 antibodyMGC5540 antibodyOTTHUMP00000237000 antibodyOTTHUMP00000237001 antibodyOTTHUMP00000237007 antibodyOTTHUMP00000237009 antibodyOTTHUMP00000237010 antibodyPP14212 antibody

  see all

Anti-ASB8 antibody images

• 

  Western blot - ASB8 antibody (ab67895)
  All lanes : Anti-ASB8 antibody (ab67895) at 1/500 dilution
  
  Lane 1 : ASB8 transfected 293T cell lysate
  Lane 2 : non transfected 293T cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
  
  Predicted band size : 32 kDa
  Observed band size : 35 kDa (why is the actual band size different from the predicted?)