Overview

  • Product nameAnti-ASC-2 antibodySee all ASC-2 primary antibodies ...
  • Description
    Rabbit polyclonal to ASC-2
  • SpecificityThis antibody specifically recognizes a 220kDa protein in U937 lysates that is blocked by the addition of the immunizing peptide; this also results in a nonspecific, lower molecular weight smear on the western blot. This antibody was tested on HEK293, HeLa, human brain and human testis extracts in western blot analysis and did not detect ASC-2 in these extracts.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human ASC-2.

  • Positive control
    • This antibody gives a positive signal in U937 lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab18193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 220 kDa in U937 extract (predicted molecular weight: 220 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe protein encoded by this gene is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone dependent fashion. It is involved in the coactivation of different nuclear receptors, such as steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). It probably functions as a general coactivator, rather than just a nuclear receptor coactivator,and may also be involved in the coactivation of the NFkB pathway (referenced from swissprot).
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • Activating signal cointegrator-2 antibody
      • AIB3 antibody
      • Amplified in breast cancer-3 protein antibody
      • Cancer-amplified transcriptional coactivator ASC-2 antibody
      • NCOA6 antibody
      • Nuclear receptor coactivator 6 antibody
      • Nuclear receptor coactivator RAP250 antibody
      • PPAR-interacting protein antibody
      • Thyroid hormone receptor-binding protein antibody
      see all

    Anti-ASC-2 antibody images

    • All lanes : Anti-ASC-2 antibody (ab18193) at 1 µg/ml

      Lane 1 : 20ug U937 lysate
      Lane 2 : 20ug U937 lysate with Human ASC-2 peptide (ab19344) at 1 µg/ml


      Predicted band size : 220 kDa
      Observed band size : 220 kDa

    References for Anti-ASC-2 antibody (ab18193)

    ab18193 has not yet been referenced specifically in any publications.

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