Overview

  • Product nameAnti-ASC-2 antibody
    See all ASC-2 primary antibodies
  • Description
    Rabbit polyclonal to ASC-2
  • SpecificityThis antibody was tested on U937, human prostate, human ovary and human testis extracts in western blot analysis and did not detect ASC-2 in these extracts.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human ASC-2.

  • Positive control
    • This antibody gives a positive signal in HeLa, HepG2 and MCF7 nuclear lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab18193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).Can be blocked with Human ASC-2 peptide (ab19344).

Target

  • RelevanceThe protein encoded by this gene is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone dependent fashion. It is involved in the coactivation of different nuclear receptors, such as steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). It probably functions as a general coactivator, rather than just a nuclear receptor coactivator,and may also be involved in the coactivation of the NFkB pathway (referenced from swissprot).
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Activating signal cointegrator-2 antibody
    • AIB3 antibody
    • Amplified in breast cancer-3 protein antibody
    • Cancer-amplified transcriptional coactivator ASC-2 antibody
    • NCOA6 antibody
    • Nuclear receptor coactivator 6 antibody
    • Nuclear receptor coactivator RAP250 antibody
    • PPAR-interacting protein antibody
    • Thyroid hormone receptor-binding protein antibody
    see all

Anti-ASC-2 antibody images

  • All lanes : Anti-ASC-2 antibody (ab18193) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 220 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 210 kDa,65 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18193 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References for Anti-ASC-2 antibody (ab18193)

ab18193 has not yet been referenced specifically in any publications.

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