Anti-ATMIN antibody - N-terminal (ab191856)
Key features and details
- Rabbit polyclonal to ATMIN - N-terminal
- Suitable for: ICC/IF, ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATMIN antibody - N-terminal -
Description
Rabbit polyclonal to ATMIN - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ATMIN (N terminal). (19 amino acid peptide from near the amino terminus; NP_056066).
Database link: O43313 -
Positive control
- WB: 293 cell lysate. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 2.5 µg/ml.
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ICC |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 88, 72 kDa).
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Notes |
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ICC/IF
Use a concentration of 2.5 µg/ml. |
ICC
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 88, 72 kDa). |
Target
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Function
Plays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage. Involved in regulating the activity of ATM in the absence of DNA damage. May play a role in stabilizing ATM. -
Tissue specificity
Ubiquitously expressed in normal tissues and cancer cell lines with highest levels in placenta and skeletal muscle. -
Sequence similarities
Contains 2 C2H2-type zinc fingers. -
Cellular localization
Nucleus. Nuclear, in discrete foci during G1 phase. - Information by UniProt
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Database links
- Entrez Gene: 23300 Human
- Omim: 614693 Human
- SwissProt: O43313 Human
- Unigene: 16349 Human
- Unigene: 589959 Human
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Alternative names
- ASCIZ antibody
- ATM INteracting protein antibody
- ATM interactor antibody
see all
Images
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Lane 1 : Anti-ATMIN antibody - N-terminal (ab191856) at 1 µg/ml
Lane 2 : Anti-ATMIN antibody - N-terminal (ab191856) at 2 µg/ml
All lanes : 293 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 88, 72 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of A431 cells labeling ATMIN with ab191856 at 2.5 μg/ml.
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Immunocytochemical analysis of A431 cells labeling ATMIN with ab191856 at 5 μg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab191856 has not yet been referenced specifically in any publications.