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Western blot - ASCL4 antibody (ab81150)
(enlarge)
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Product Name
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ASCL4 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ASCL4
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Immunogen
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Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MMETRKPAER LALPYSLRTA PLGVPGTLPG LPRRDPLRVA LRLDAACWEW) of Human ASCL4 (NP_982260).
BLAST 'MMETRKPAER LALPYSLRTA PLGVPGTLPG LPRRDPLRVA LRLDAACWEW ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA titre using peptide based assay 1/312500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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MCF7 cell lysate
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Cellular localization
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Nucleus
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
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Relevance
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ASCL4 is a basic helix-loop-helix transcription factor. It is essential for the determination of cell fate and the development and differentiation of numerous tissues. It could be a transcriptional regulator involved in skin development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ASCL4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ASCL4 antibody images, references, products related to ab81150 and other tools.
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ASCL4 antibody images:
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 Western blot - ASCL4 antibody (ab81150)
ASCL4 antibody (ab81150) at 1 µg/ml + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 19 kDa
Observed band size : 19 kDa
Gel concentration 12%
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Search PubMed (MEDLINE) for references to ASCL4
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ASCL4 antibody - more information
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Customer reviews (feedback) regarding ASCL4 antibody |
Customer FAQs regarding ASCL4 antibody |
Protocols for ASCL4 antibody |
Price and availability of products related to ASCL4 antibody |
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ASCL4 antibody - related products:
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ASCL4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ASCL4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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