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Western blot - ASF1 alpha/beta antibody (ab53608)
(enlarge)
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Product Name
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ASF1 A+B antibody
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See all ASF1 A+B antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ASF1 A+B
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Immunogen
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Recombinant full length protein (Fruit fly (Drosophila melanogaster))
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Reacts with
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Dm
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Tested applications
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/200.
IP: 1/500.
WB: 1/2000. Predicted molecular weight: 24 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
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Relevance
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Asf1 (anti silencing function 1) belongs to the H3/H4 family of histone chaperones. Its function has not been fully elucidated but it is believed to be involved in ensuring a constant supply of histones at sites of nucleosome assembly. Asf1 interacts with the DNA damage checkpoint protein Rad53 and so may have a role in cell cycle regulation and DNA responses. ASF1 A and B (the two human subtypes) are the only known substrate for Tousled like kinase (TLK).
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ASF1 A+B antibody (ab53608) |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol, Whole serum Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ASF1 A+B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ASF1 A+B antibody images, references, products related to ab53608 and other tools.
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ASF1 A+B antibody images:
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 Western blot - ASF1 alpha/beta antibody (ab53608)
All lanes : ASF1 A+B antibody (ab53608) at 1/2000 dilution
Lane 1 : Drosophila embryo lysate
Lane 2 : Drosophila embryo lysate
Lane 3 : Drosophila embryo lysate
Predicted band size : 24 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
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References for ASF1 A+B antibody (ab53608)
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This product has been used in: (two most recent references)
Schulz LL & Tyler JK The histone chaperone ASF1 localizes to active DNA replication forks to mediate efficient DNA replication. FASEB J 20:488-90 (2006). PubMed: 16396992
Tyler JK et al. Interaction between the Drosophila CAF-1 and ASF1 chromatin assembly factors. Mol Cell Biol 21:6574-84 (2001). PubMed: 11533245
See all 3 publication references for this product.
If you publish research using ab53608 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ASF1 A+B
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ASF1 A+B antibody - more information
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Customer reviews (feedback) regarding ASF1 A+B antibody |
Customer FAQs regarding ASF1 A+B antibody |
Protocols for ASF1 A+B antibody |
Price and availability of products related to ASF1 A+B antibody |
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ASF1 A+B antibody - related products:
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ASF1 A+B antibody - other tools:
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Contact Abcam with a Technical Enquiry about ASF1 A+B antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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