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Western blot - ASF1a antibody (ab22231)
(enlarge)
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Product Name
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ASF1A antibody
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See all ASF1A antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to ASF1A
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Immunogen
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Fusion protein: RFHINWEDNTEKLEDAESSN, corresponding to amino acids 148/167 of Human ASF1a
BLAST 'RFHINWEDNTEKLEDAESSN' with  or 
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Reacts with
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Hu
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Predicted to react
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Ms, Chk, Cow
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 23 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
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Relevance
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ASF1a belongs to the H3/H4 family of histone chaperone proteins. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication, repair and silencing.
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Constituents: 50% Glycerol Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Notes
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Produced from outbred CD1 mice
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ASF1A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ASF1A antibody images, references, products related to ab22231 and other tools.
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ASF1A antibody images:
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 Western blot - ASF1a antibody (ab22231)
All lanes : ASF1A antibody (ab22231) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 23 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
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Search PubMed (MEDLINE) for references to ASF1A
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ASF1A antibody - more information
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Customer reviews (feedback) regarding ASF1A antibody |
Customer FAQs regarding ASF1A antibody |
Protocols for ASF1A antibody |
Price and availability of products related to ASF1A antibody |
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ASF1A antibody - related products:
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ASF1A antibody - other tools:
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Contact Abcam with a Technical Enquiry about ASF1A antibody |
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