You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-ASF1A antibody (ab22231)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-ASF1A antibody
See all ASF1A products (5) ...

Description

Mouse polyclonal to ASF1A

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Chicken, Cow

Immunogen

Fusion protein: RFHINWEDNTEKLEDAESSN, corresponding to amino acids 148/167 of Human ASF1a

RFHINWEDNT EKLEDAESSN

General notes

Produced from outbred CD1 mice

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Constituents: 50% Glycerol

Purity

Whole antiserum

Primary antibody notes

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - ASF1a antibody (ab22231)Western blot - ASF1a antibody (ab22231) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab22231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: 1/1000. Predicted molecular weight: 23 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

Function

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Tissue specificity

Ubiquitously expressed.

Sequence similarities

Belongs to the ASF1 family.

Post-translational
modifications

Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9Y294 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Anti silencing function 1A antibody
  • Anti-silencing function protein 1 homolog A antibody
  • ASF1 anti silencing function 1 homolog A antibody
  • asf1a antibody
  • ASF1A_HUMAN antibody
  • CCG1 interacting factor A antibody
  • CCG1-interacting factor A antibody
  • CGI 98 antibody
  • CGI 98 protein antibody
  • CIA antibody
  • hAsf1 antibody
  • hAsf1a antibody
  • hCIA antibody
  • Histone chaperone ASF1A antibody
  • HSPC146 antibody
  • Hypothetical protein DKFZp564E2182 antibody
  • Hypothetical protein FLJ22085 antibody
see all

Anti-ASF1A antibody images:

  Western blot - ASF1a antibody (ab22231)

Western blot - ASF1a antibody (ab22231)

All lanes : Anti-ASF1A antibody (ab22231) at 1/1000 dilution

Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

Predicted band size : 23 kDa


The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.

References for Anti-ASF1A antibody (ab22231)

ab22231 has not yet been referenced specifically in any publications.

Publishing research using ab22231? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"