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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab66754 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - ASF1a antibody (ab66754)
All lanes : Anti-ASF1A antibody (ab66754) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Additional bands at : 39 kDa,73 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
The 21 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to ASF1a.
ASF1A antibody for IHC-P in Human (66754)
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