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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Overview
Product name
Anti-ASF1A antibody
See all ASF1A products (5) ...
Description
Rabbit polyclonal to ASF1A
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Chicken
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human ASF1A.
(Peptide available as ab71590.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: Jurkat, Staurosporine-Treated Jurkat (24hr, 500nM)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - ASF1a antibody (ab66754)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
Target
Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the ASF1 family.
Post-translational
modifications
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9Y294
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Anti silencing function 1A antibody
- Anti-silencing function protein 1 homolog A antibody
- ASF1 anti silencing function 1 homolog A antibody
see all
Database links
- Entrez Gene: 723780 Chicken
- Entrez Gene: 25842 Human
- Entrez Gene: 66403 Mouse
- Omim: 609189 Human
- SwissProt: Q9Y294 Human
- SwissProt: Q9CQE6 Mouse
- Unigene: 1997 Chicken
- Unigene: 292316 Human
see all
Anti-ASF1A antibody images:
Western blot - ASF1a antibody (ab66754)
All lanes : Anti-ASF1A antibody (ab66754) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Additional bands at : 39 kDa,73 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
The 21 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to ASF1a.
References for Anti-ASF1A antibody (ab66754)
ab66754 has not yet been referenced specifically in any publications.
Publishing research using ab66754? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"