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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Overview
Product name
Anti-ASF1a antibody
See all ASF1A products (5) ...
Description
Goat polyclonal to ASF1a
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Chicken, Cow, Xenopus laevis
Immunogen
Synthetic peptide: ENSLNVMLESH, from the C Terminus of Human ASF1a
ENSLNVMLES H
Positive control
Jurkat cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - ASF1a antibody (ab65942)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
Peptide ELISA: antibody detection limit dilution 1:32,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the ASF1 family.
Post-translational
modifications
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9Y294
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Anti silencing function 1A antibody
- Anti-silencing function protein 1 homolog A antibody
- ASF1 anti silencing function 1 homolog A antibody
see all
Database links
- Entrez Gene: 723780 Chicken
- Entrez Gene: 618099 Cow
- Entrez Gene: 25842 Human
- Entrez Gene: 66403 Mouse
- Entrez Gene: 380002 Xenopus laevis
- Omim: 609189 Human
- SwissProt: Q2KIG1 Cow
- SwissProt: Q9Y294 Human
see all
Anti-ASF1a antibody images:
Western blot - ASF1a antibody (ab65942)
Anti-ASF1a antibody (ab65942) at 1 µg/ml + Jurkat cell lysate at 35 µg
Predicted band size : 23 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
References for Anti-ASF1a antibody (ab65942)
ab65942 has not yet been referenced specifically in any publications.
Publishing research using ab65942? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"