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Western blot - ASF1a antibody (ab65942)
(enlarge)
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Product Name
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ASF1a antibody
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See all ASF1A antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ASF1a
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Immunogen
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Synthetic peptide: ENSLNVMLESH, from the C Terminus of Human ASF1a
BLAST 'ENSLNVMLESH' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Chk, Cow, Xl
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
Peptide ELISA: antibody detection limit dilution 1:32,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cells.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
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Relevance
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ASF1a belongs to the H3/H4 family of histone chaperone proteins. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication, repair and silencing.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ASF1a antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ASF1a antibody images, references, products related to ab65942 and other tools.
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ASF1a antibody images:
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Search PubMed (MEDLINE) for references to ASF1A
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ASF1a antibody - more information
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Customer reviews (feedback) regarding ASF1a antibody |
Customer FAQs regarding ASF1a antibody |
Protocols for ASF1a antibody |
Price and availability of products related to ASF1a antibody |
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ASF1a antibody - related products:
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ASF1a antibody - other tools:
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Contact Abcam with a Technical Enquiry about ASF1a antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - ASF1a antibody (ab65942)