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Anti-ASF1a antibody (ab69935)

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Overview

Product name

Anti-ASF1a antibody
See all ASF1A products (5) ...

Description

Rabbit polyclonal to ASF1a

Specificity

This antibody may cross-react with ASF1 beta.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide: SNPRVTRFH INWEDNTEKL EDAESSNPN L, corresponding to internal sequence amino acids 141-170 of Human ASF1a

SNPRVTRFH INWEDNTEKL EDAESSNPNL

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Jurkat cell extract

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other

  • Western blot - ASF1a antibody (ab69935)Western blot - ASF1a antibody (ab69935) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab69935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

Target

Function

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Tissue specificity

Ubiquitously expressed.

Sequence similarities

Belongs to the ASF1 family.

Post-translational
modifications

Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9Y294 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Anti silencing function 1A antibody
  • Anti-silencing function protein 1 homolog A antibody
  • ASF1 anti silencing function 1 homolog A antibody
  • asf1a antibody
  • ASF1A_HUMAN antibody
  • CCG1 interacting factor A antibody
  • CCG1-interacting factor A antibody
  • CGI 98 antibody
  • CGI 98 protein antibody
  • CGI98 antibody
  • CIA antibody
  • hAsf1 antibody
  • hAsf1a antibody
  • hCIA antibody
  • Histone chaperone ASF1A antibody
  • HSPC146 antibody
  • Hypothetical protein FLJ22085 antibody
see all

Database links

Anti-ASF1a antibody images:

  Western blot - ASF1a antibody (ab69935)

Western blot - ASF1a antibody (ab69935)

All lanes : Anti-ASF1a antibody (ab69935) at 1/500 dilution

Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 23 kDa
Observed band size : 23 kDa

References for Anti-ASF1a antibody (ab69935)

ab69935 has not yet been referenced specifically in any publications.

Publishing research using ab69935? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"