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- Secondary antibodies
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Product name
Anti-ASH2L antibody
See all ASH2L products (6) ...
Description
Rabbit polyclonal to ASH2L
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow, Dog
Immunogen
Synthetic peptide derived from residues 54-103 of human ASH2L.
Positive control
Jurkat cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Trithorax
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
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Western blot - ASH2L antibody (ab28426)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/12500.ELISA: 1/12500.
WB: Use a concentration of 0.25 µg/ml. Predicted molecular weight: 69 kDa.
Target
Function
Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
Tissue specificity
Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
Sequence similarities
Contains 1 B30.2/SPRY domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBL3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ash2 (absent small or homeotic) like antibody
- ASH2 like protein antibody
- ASH 2 antibody
see all
Database links
- Entrez Gene: 9070 Human
- Entrez Gene: 23808 Mouse
- Entrez Gene: 290829 Rat
- Omim: 604782 Human
- SwissProt: Q9UBL3 Human
- SwissProt: Q3UIF9 Mouse
- SwissProt: Q91X20 Mouse
- Unigene: 521530 Human
see all
Anti-ASH2L antibody images:
Western blot - ASH2L antibody (ab28426)
Anti-ASH2L antibody (ab28426) at 0.25 µg/ml + Jurkat cell lysate
Secondary
HRP-conjugated anti rabbit IgG diluted 1/50,000 - 1/100,000
Predicted band size : 69 kDa
References for Anti-ASH2L antibody (ab28426)
This product has been referenced in:
- De Santa F et al. The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing. Cell 130:1083-94 (2007). WB; Human, Mouse.Read more (PubMed: 17825402) »
See 1 publication for this product
Publishing research using ab28426? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"