Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
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ab45178 has been referenced in 4 publications.
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Anti-ASK1 antibody [EP553Y] (ab45178) at 1/2000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat anti-rabbit, HRP labelled at 1/2000 dilution
Predicted band size : 155 kDa
Observed band size : 155 kDa
Ab45178 (1/10) staining human ASK1 in human lung carcinoma by immunohistochemistry using paraffin embedded tissue.
Immunofluorescent staining of HeLa cells using ab45178 (1/10).
Overlay histogram showing HeLa cells stained with ab45178 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45178, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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