Anti-ASK1 antibody [EP553Y] (ab45178)

The lot# is GR48990-3. Please let me know the protein concentration in this lot antibody.

Could you also give the quote for 10 vials antibody with lot# GR48990-3? Thanks.

ANSWER:

The concentration of ab45178, lot GR48990-3, is 1.262 mg/mL.

I have CC's your request for a quote for 10 vials of this lot to our bulk order specialist, who can be reached at sales@abcam.com. She will be able to provide you a quote which includes a discount for purchasing at least 10 vials.

I hope this helps. Please let me know if you have any further questions.

Thanks for the information. This antibody has not protein concentration. How do you determine the protein concentration for isotype control?

ANSWER:

Thanks for your enquiry.

If you could send along the lot number Iwill be able to provide the antibody concentration.

Could you kindly provide your ICC staining protocol using anti-ASK1 antibody (ab45178)? How do you do antigen retrieval?

Thanks.

ANSWER:

Thank you for your enquiry.

I think the recommendation to use antigen retrieval listed for ICC/IFis a mistake. Antigen retrieval should not be necessary for ICC. Here is a link to a good general ICC protocol on our website if you need it:

http://www.abcam.com/index.html?pageconfig=resource&rid=11417

I hope this is helpful. Please contact me again if you have any further questions.

Could you let me know the cat# for isotope control? Thanks.

ANSWER:

Thank you for your enquiry.

The rabbit monoclonal isotype control antibody that was used in this flow experiment is not available on our catalog. We do have a rabbit polyclonal isotype control, http://www.abcam.com/Rabbit-polyclonal-IgG-ab27478.html, which would still be a reasonable control.

I hope this is helpful. Please contact us again if you have any further questions.

I am interested in using your anti-ASK1 antibody (ab45178) for FACS analysis. Could you kindly provide your protocol? Thanks.



Saili yi

ANSWER:

Thank you for your enquiry.

On our online datasheet for ab45178, there is a FACS figure with the accompanying figure legend:

"Overlay histogram showing HeLa cells stained with ab45178 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45178, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed."

Also, there is 1 Abreview regarding Flow cyt that may also contain some useful protocol information.

Both details above can be found on the online product datasheet at: http://www.abcam.com/ASK1-antibody-EP553Y-ab45178.html

I hope this is helpful. Please contact me if you have any further questions.