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Western blot - ASK1 antibody (ab2004)
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Immunocytochemistry/ Immunofluorescence - ASK1 antibody (ab2004)
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Product Name
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ASK1 antibody
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See all ASK1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ASK1
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Immunogen
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Peptide sequence corresponding to amino acids 1356 to 1375 ofhuman ASK1 (1).
This sequence is different from that of mouse by last two amino acids (3).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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SW1353, A431 and K-562 whole cell lysates
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Cellular localization
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Cytoplasmic
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Apoptosis
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Relevance
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Mitogen activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1). ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas associated protein Daxx. Overexpression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibits TNF alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Apoptosis Signal-regulation Kinase
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ASK1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ASK1 antibody images, references, products related to ab2004 and other tools.
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ASK1 antibody images:
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 Western blot - ASK1 antibody (ab2004)
ASK1 antibody (ab2004) at 1/500 dilution + SW1351 whole cell lysate
Predicted band size : 155 kDa
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Immunocytochemistry/ Immunofluorescence - ASK1 antibody (ab2004)
ab2004 at 10µg/ml staining ASK1 in A431 cells by ICC/IF
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Search PubMed (MEDLINE) for references to ASK1
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ASK1 antibody - more information
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Customer reviews (feedback) regarding ASK1 antibody |
Customer FAQs regarding ASK1 antibody |
Protocols for ASK1 antibody |
Price and availability of products related to ASK1 antibody |
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ASK1 antibody - related products:
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ASK1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ASK1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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