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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-ASK1 antibody
See all ASK1 products (13) ...
Description
Rabbit polyclonal to ASK1
Tested applications
IP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Peptide sequence corresponding to amino acids 1356 to 1375 ofhuman ASK1 (1). This sequence is different from that of mouse by last two amino acids (3).
Positive control
SW1353, A431 and K-562 whole cell lysates
General notes
Apoptosis Signal-regulation Kinase
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Apoptosis
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. (PubMed: 12424221)
WB: 1/500 - 1/1000.Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
Tissue specificity
Abundantly expressed in heart and pancreas.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Dephosphorylated and activated by PGAM5.
Target information above from: UniProt accessionQ99683
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis signal regulating kinase 1 antibody
- Apoptosis signal-regulating kinase 1 antibody
- ASK 1 antibody
see all
Database links
- Entrez Gene: 4217 Human
- Omim: 602448 Human
- SwissProt: Q99683 Human
- Unigene: 186486 Human
Anti-ASK1 antibody images:
Western blot - ASK1 antibody (ab2004)
Anti-ASK1 antibody (ab2004) at 1/500 dilution + SW1351 whole cell lysate
Predicted band size : 155 kDa
Immunocytochemistry/ Immunofluorescence - ASK1 antibody (ab2004)
ab2004 at 10µg/ml staining ASK1 in A431 cells by ICC/IF
References for Anti-ASK1 antibody (ab2004)
This product has been referenced in:
- Filomeni Get al. Glutathione disulfide induces apoptosis in U937 cells by a redox-mediated p38 MAP kinase pathway. FASEB J 17:64-6 (2003). WB, IP; Human.Read more (PubMed: 12424221) »
See 1 publication for this product
Publishing research using ab2004? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"