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Product name
Anti-ASK1 antibody
See all ASK1 products (14) ...
Description
Rabbit polyclonal to ASK1
Specificity
ab39650 detects endogenous levels of total ASK1 protein.
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide (non-phosphorylated) derived from Human ASK1 around the phosphorylation site of Serine 966.
Positive control
Human breast carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Apoptosis
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Immunohistochemistry (Paraffin-embedded sections) - ASK1 antibody (ab39650)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
Target
Function
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
Tissue specificity
Abundantly expressed in heart and pancreas.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Dephosphorylated and activated by PGAM5.
Target information above from: UniProt accessionQ99683
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis signal regulating kinase 1 antibody
- Apoptosis signal-regulating kinase 1 antibody
- ASK 1 antibody
see all
Database links
- Entrez Gene: 4217 Human
- Entrez Gene: 26408 Mouse
- Omim: 602448 Human
- SwissProt: Q99683 Human
- SwissProt: O35099 Mouse
- Unigene: 186486 Human
- Unigene: 6595 Mouse
Anti-ASK1 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - ASK1 antibody (ab39650)
ab39650, at 1/50 dilution, staining ASK1 in Human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded tissue. Left: sample not treated with peptide. Right: sample treated with synthetic peptide.
References for Anti-ASK1 antibody (ab39650)
ab39650 has not yet been referenced specifically in any publications.
Publishing research using ab39650? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"