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Product name
Anti-ASK1 (phospho S966) antibody
See all ASK1 products (14) ...
Description
Rabbit polyclonal to ASK1 (phospho S966)
Specificity
ab39402 detects endogenous levels of ASK1 only when phosphorylated at Serine 966.
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide: SISpLP, derived from Human ASK1 around the phosphorylation site of Serine 966
SISpLP
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab39402 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Apoptosis
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
Target
Function
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
Tissue specificity
Abundantly expressed in heart and pancreas.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Dephosphorylated and activated by PGAM5.
Target information above from: UniProt accessionQ99683
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis signal regulating kinase 1 antibody
- Apoptosis signal-regulating kinase 1 antibody
- ASK 1 antibody
see all
Database links
- Entrez Gene: 4217 Human
- Entrez Gene: 26408 Mouse
- Omim: 602448 Human
- SwissProt: Q99683 Human
- SwissProt: O35099 Mouse
- Unigene: 186486 Human
- Unigene: 6595 Mouse
Anti-ASK1 (phospho S966) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - ASK1 (phospho S996) antibody (ab39402)
ab39402, at a 1/100 dilution, staining human ASK1 in breast carcinoma tissue by Immunohistochemistry, Paraffin embedded tissue.
Immunohistochemistry (Paraffin-embedded sections) - ASK1 (phospho S996) antibody (ab39402)
ab39402, at a 1/100 dilution, staining human ASK1 in breast carcinoma tissue (treated with synthesized phosphopeptide) by Immunohistochemistry, Paraffin embedded tissue.
References for Anti-ASK1 (phospho S966) antibody (ab39402)
ab39402 has not yet been referenced specifically in any publications.
Publishing research using ab39402? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"