Anti-ASS1 antibody (ab77590)
Key features and details
- Goat polyclonal to ASS1
- Suitable for: ICC/IF, IHC-P, Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ASS1 antibody
See all ASS1 primary antibodies -
Description
Goat polyclonal to ASS1 -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, IHC-P, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog, Orangutan -
Immunogen
Synthetic peptide:
ENPKNQAPPGLYTKTQD
(Human) from the internal region of the protein sequence according to NP_000041.2 ; NP_446464.1. -
Positive control
- ICC: HeLa cells. WB: A431 and NIH/3T3 cell lysate. Human and rat kidney lysate. Mouse liver lysate. Flow Cyt (intra): A431 cells. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Pathway
Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1. -
Involvement in disease
Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood. -
Sequence similarities
Belongs to the argininosuccinate synthase family. Type 1 subfamily. - Information by UniProt
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Database links
- Entrez Gene: 445 Human
- Entrez Gene: 11898 Mouse
- Entrez Gene: 25698 Rat
- Omim: 603470 Human
- SwissProt: P00966 Human
- SwissProt: P16460 Mouse
- SwissProt: P09034 Rat
- Unigene: 160786 Human
see all -
Alternative names
- Argininosuccinate synthase 1 antibody
- Argininosuccinate synthase antibody
- Argininosuccinate synthetase 1 antibody
see all
Images
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Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab77590 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Lane 1 : Anti-ASS1 antibody (ab77590) at 0.3 µg/ml
Lane 2 : Anti-ASS1 antibody (ab77590) at 1 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 47 kDa -
Lanes 1-2 : Anti-ASS1 antibody (ab77590) at 0.01 µg/ml
Lane 3 : Anti-ASS1 antibody (ab77590) at 0.03 µg/ml
Lane 1 : Human Kidney
Lane 2 : Mouse Liver
Lane 3 : Rat Kidney
Lysates/proteins at 35 µg per lane.
Predicted band size: 47 kDa -
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab77590 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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ICC/IF image of ab77590 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween (no animal sera) for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77590 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- goat (ab150133) IgG used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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IHC image of ab77590 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77590, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab77590 has been referenced in 5 publications.
- Miura A et al. Macrophage potentiates the recovery of liver zonation and metabolic function after acute liver injury. Sci Rep 11:9730 (2021). PubMed: 33958644
- Aboulouard S et al. In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer. Cell Rep Med 2:100318 (2021). PubMed: 34195683
- Drokhlyansky E et al. The Human and Mouse Enteric Nervous System at Single-Cell Resolution. Cell 182:1606-1622.e23 (2020). PubMed: 32888429
- Cunningham SC et al. Modeling correction of severe urea cycle defects in the growing murine liver using a hybrid recombinant adeno-associated virus/piggyBac transposase gene delivery system. Hepatology 62:417-28 (2015). PubMed: 26011400
- Kok CY et al. Adeno-associated Virus-mediated Rescue of Neonatal Lethality in Argininosuccinate Synthetase-deficient Mice. Mol Ther 21:1823-31 (2013). PubMed: 23817206