Anti-ASXL1 antibody [2049C2a] (ab50817)
Key features and details
- Mouse monoclonal [2049C2a] to ASXL1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-ASXL1 antibody [2049C2a]
See all ASXL1 primary antibodies -
Description
Mouse monoclonal [2049C2a] to ASXL1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment of ASXL1 (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
2049C2a -
Isotype
IgG1 -
Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution. Predicted molecular weight: 165 kDa.
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Notes |
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WB
Use at an assay dependent dilution. Predicted molecular weight: 165 kDa. |
Target
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Function
Probable Polycomb group (PcG) protein. Non-catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). -
Tissue specificity
Widely expressed at low level. Expressed in heart, brain, skeletal muscle, placenta, pancreas, spleen, prostate, small intestine, colon, peripheral blood, leukocytes, bone marrow and fetal liver. Highly expressed in testes. -
Sequence similarities
Belongs to the Asx family.
Contains 1 PHD-type zinc finger. -
Domain
Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which may be required for an association with nuclear receptors. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- Additional sex combs like 1 (Drosophila) antibody
- Additional sex combs like protein 1 antibody
- Additional sex combs-like protein 1 antibody
see all
Images
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Anti-ASXL1 antibody [2049C2a] (ab50817) + Immunizing recombinant fragment
Predicted band size: 165 kDa
Observed band size: 39,43 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab50817 has been referenced in 1 publication.
- Balasubramani A et al. Cancer-associated ASXL1 mutations may act as gain-of-function mutations of the ASXL1-BAP1 complex. Nat Commun 6:7307 (2015). WB ; Mouse . PubMed: 26095772