Anti-ATAD3A antibody (ab67992)
Key features and details
- Mouse polyclonal to ATAD3A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATAD3A antibody
See all ATAD3A primary antibodies -
Description
Mouse polyclonal to ATAD3A -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human ATAD3A aa 1-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Spleen tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
Target
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Relevance
ATAD3A belongs to the AAA ATPase family. ATPases are a class of enzymes that catalyze the decomposition of adenosine triphosphate (ATP) into adenosine diphosphate (ADP) and a free phosphate ion. Two different isoforms obtained by alternative splicing, are known. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 55210 Human
- Omim: 612316 Human
- SwissProt: Q9NVI7 Human
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Alternative names
- AAA-ATPase TOB3 antibody
- Atad3a antibody
- ATPase family AAA domain containing protein 3A antibody
see all
Images
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Anti-ATAD3A antibody (ab67992) at 1/500 dilution + Human spleen tissue lysate at 25 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugated at 1/2500 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted? -
All lanes : Anti-ATAD3A antibody (ab67992) at 1/500 dilution
Lane 1 : ATAD3A transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab67992 has been referenced in 1 publication.
- Hescot S et al. Identifying mitotane-induced mitochondria-associated membranes dysfunctions: metabolomic and lipidomic approaches. Oncotarget 8:109924-109940 (2017). PubMed: 29299119