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Product Name
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ATEB1B antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ATEB1B
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Immunogen
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Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids 205-221 of Arabidopsis thaliana ATEB1B
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Reacts with
(species key)
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At
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Specificity
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Recognizes AtEB1b fusion protein expressed in transgenic Arabidopsis plants. The immunogen sequence is highly conserved (single amino acid substitution) in AtEB1a and AtEB1c.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
An additional band at 50 kDa may be observed representing AtEB1b degradation products. Staining of AtEB1b in immunoblotting is specifically inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extract (cytosolic fraction) from the leaves of transgenic Arabidopsis seedlings expressing YFP-taged AtEB1b.
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Cellular localization
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Cytoskeleton
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Research areas
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Cell Biology >> Other Antibodies >> Plant related targets
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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Relevance
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Microtubules (MT) play a central role in many cellular functions including: intracellular transport, maintenance of cell shape and polarity, and chromosome segregation. In most cells, the minus-ends of microtubules are anchored at the centrosome, whereas plus-ends are more dynamic and responsible for contacting targets such as cell cortex and chromosomes. Several factors regulate MT dynamics in cells. EB1 (End-Binding protein 1) proteins belong to a distinct family of accessory proteins, termed "plus-end tracking proteins" or "+TIPs", that bind to the
plus-ends of microtubules and are thought to play an important role in regulating dynamics and organization of microtubules. These microtubule-associated proteins (MAPs) are among the most conserved components of the MT cytoskeleton. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.
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ATEB1B antibody (ab50187) |
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 836370 (Arabidopsis thaliana)
823923 (Arabidopsis thaliana)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab50187 is affinity-purified using the immunizing peptide immobilized on agarose.
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATEB1B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to AtEB1B
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ATEB1B antibody - more information
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Customer reviews (feedback) regarding ATEB1B antibody |
Customer FAQs regarding ATEB1B antibody |
Protocols for ATEB1B antibody |
Price and availability of products related to ATEB1B antibody |
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