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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Overview
Product name
Anti-ATEB1B antibody
Description
Rabbit polyclonal to ATEB1B
Specificity
Recognizes AtEB1b fusion protein expressed in transgenic Arabidopsis plants. The immunogen sequence is highly conserved (single amino acid substitution) in AtEB1a and AtEB1c.
Tested applications
Cross reactivity
Reacts with
Arabidopsis thaliana
Immunogen
Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids 205-221 of Arabidopsis thaliana ATEB1B
Positive control
Extract (cytosolic fraction) from the leaves of transgenic Arabidopsis seedlings expressing YFP-taged AtEB1b.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab50187 is affinity-purified using the immunizing peptide immobilized on agarose.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Plant related targets
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
An additional band at 50 kDa may be observed representing AtEB1b degradation products. Staining of AtEB1b in immunoblotting is specifically inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Microtubules (MT) play a central role in many cellular functions including: intracellular transport, maintenance of cell shape and polarity, and chromosome segregation. In most cells, the minus-ends of microtubules are anchored at the centrosome, whereas plus-ends are more dynamic and responsible for contacting targets such as cell cortex and chromosomes. Several factors regulate MT dynamics in cells. EB1 (End-Binding protein 1) proteins belong to a distinct family of accessory proteins, termed "plus-end tracking proteins" or "+TIPs", that bind to the plus-ends of microtubules and are thought to play an important role in regulating dynamics and organization of microtubules. These microtubule-associated proteins (MAPs) are among the most conserved components of the MT cytoskeleton. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.
Cellular localization
Cytoskeleton
Alternative names
- Arabidopsis thaliana EB1B antibody
- AT5G62500 antibody
- ATEB1A antibody
see all
Database links
- Entrez Gene: 823923 Arabidopsis thaliana
- Entrez Gene: 836370 Arabidopsis thaliana
References for Anti-ATEB1B antibody (ab50187)
ab50187 has not yet been referenced specifically in any publications.
Publishing research using ab50187? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"