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Overview

  • Product nameAnti-ATEB1B antibody
  • Description
    Rabbit polyclonal to ATEB1B
  • SpecificityRecognizes AtEB1b fusion protein expressed in transgenic Arabidopsis plants. The immunogen sequence is highly conserved (single amino acid substitution) in AtEB1a and AtEB1c.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Arabidopsis thaliana
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids 205-221 of Arabidopsis thaliana ATEB1B

  • Positive controlExtract (cytosolic fraction) from the leaves of transgenic Arabidopsis seedlings expressing YFP-taged AtEB1b.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab50187 is affinity-purified using the immunizing peptide immobilized on agarose.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab50187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
    An additional band at 50 kDa may be observed representing AtEB1b degradation products. Staining of AtEB1b in immunoblotting is specifically inhibited with the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceMicrotubules (MT) play a central role in many cellular functions including: intracellular transport, maintenance of cell shape and polarity, and chromosome segregation. In most cells, the minus-ends of microtubules are anchored at the centrosome, whereas plus-ends are more dynamic and responsible for contacting targets such as cell cortex and chromosomes. Several factors regulate MT dynamics in cells. EB1 (End-Binding protein 1) proteins belong to a distinct family of accessory proteins, termed "plus-end tracking proteins" or "+TIPs", that bind to the plus-ends of microtubules and are thought to play an important role in regulating dynamics and organization of microtubules. These microtubule-associated proteins (MAPs) are among the most conserved components of the MT cytoskeleton. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.
    • Cellular localizationCytoskeleton
    • Database links
      • Alternative names
          Arabidopsis thaliana EB1B antibodyAT5G62500 antibodyATEB1A antibody
          AtEB1B antibodyK19B1.11 antibodyK19B1_11 antibodymicrotubule binding antibody
        see all

      References for Anti-ATEB1B antibody (ab50187)

      ab50187 has not yet been referenced specifically in any publications.

      Publishing research using ab50187 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"