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Western blot - ATF1 antibody (ab52845)
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Product Name
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ATF1 antibody
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See all ATF1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to ATF1
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Immunogen
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Fusion protein: MEDSHKSNTT ETASQPGSTV AGPHVSQIVH QVSSLSESEE SQDSSDSIGS SQKAHGILAR RPSYRKILKD LSSEDTRGRK GEGENPSISA ITSMSVPAPI , corresponding to amino acids 1-100 of Mouse ATF1
BLAST 'MEDSHKSNTT ETASQPGSTV AGPHVSQIVH QVSSLSESEE SQDSSDSIGS SQKAHGILAR RPSYRKILKD LSSEDTRGRK GEGENPSISA ITSMSVPAPI ' with  or 
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Reacts with
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Ms
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 29 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Relevance
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ATF1 (Activating Transcription Factor 1, TREB-36) is a member of the ATF/CREB family of basic region leucine-zipper (bZip) DNA-binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. The activation function of CRE-binding proteins may be modulated by phosphorylation of several kinases and is mediated by coactivators such as CREB-binding protein (CBP) and p300. ATF1 is a nuclear protein that binds DNA as a homodimer or as heterodimers with the inducible transcription factors CREB1 or CREM. Heterodimers appear to be stronger transcriptional activators than the homodimers. Tissue expression of ATF1 mRNA is widespread. Several isoforms of ATF1 arise by differential splicing. ATF1 mediates both Ca2+ and cAMP responses at several levels. It binds to the Tax-responsive element (TRE1) of the human T-cell lymphotropic virus type-I (HTLV1). ATF1 is detectable in metastatic melanoma cells and seems to contribute to their survival. A chimeric protein composed of the N-terminal domain of EWS (Ewing sarcoma oncogene) linked to the bZip domain of ATF1 is implicated in the rare malignant clear cell sarcoma of tendon sheath and aponeuroses (malignant melanoma of soft parts).
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 11908 (Mouse)
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol, Whole serum Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATF1 antibody images, references, products related to ab52845 and other tools.
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ATF1 antibody images:
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 Western blot - ATF1 antibody (ab52845)
All lanes : ATF1 antibody (ab52845) at 1/1000 dilution
Lane 1 : Total protein extract from E coli with ~50ng to 100 ng of an irrelevant antigen at 20 µg
Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen at 20 µg
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 29 kDa
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is tagged
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Search PubMed (MEDLINE) for references to ATF1
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ATF1 antibody - more information
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