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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-ATF1 antibody
See all ATF1 products (8) ...
Description
Mouse polyclonal to ATF1
Tested applications
Cross reactivity
Reacts with
Mouse
Immunogen
Fusion protein: MEDSHKSNTT ETASQPGSTV AGPHVSQIVH QVSSLSESEE SQDSSDSIGS SQKAHGILAR RPSYRKILKD LSSEDTRGRK GEGENPSISA ITSMSVPAPI , corresponding to amino acids 1-100 of Mouse ATF1
MEDSHKSNTT ETASQPGSTV AGPHVSQIVH QVSSLSESEE SQDSSDSIGS SQKAHGILAR RPSYRKILKD LSSEDTRGRK GEGENPSISA ITSMSVPAPI
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Western blot - ATF1 antibody (ab52845)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 29 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
Involvement in disease
Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP18846
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- TREB36 protein antibody
- Activating transcription factor 1 antibody
- ATF 1 antibody
see all
Database links
- Entrez Gene: 11908 Mouse
- SwissProt: P81269 Mouse
- Unigene: 473546 Mouse
- Unigene: 676 Mouse
Anti-ATF1 antibody images:
Western blot - ATF1 antibody (ab52845)
All lanes : Anti-ATF1 antibody (ab52845) at 1/1000 dilution
Lane 1 : Total protein extract from E coli with ~50ng to 100 ng of an irrelevant antigen at 20 µg
Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen at 20 µg
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 29 kDa
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is tagged
References for Anti-ATF1 antibody (ab52845)
ab52845 has not yet been referenced specifically in any publications.
Publishing research using ab52845? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"