Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-ATF1 antibody
See all ATF1 products (8) ...
Description
Rabbit polyclonal to ATF1
Specificity
ab66016 detects endogenous levels of total ATF1 protein.
Tested applications
ELISA, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from an internal sequence of human ATF1.
Positive control
ICC/IF: HUVEC cells WB: Extracts from COLO205 cells, HeLa cells and HepG2 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab66016 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000.Detects a band of approximately 40 kDa (predicted molecular weight: 29 kDa).
Target
Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
Involvement in disease
Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP18846
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- TREB36 protein antibody
- Activating transcription factor 1 antibody
- ATF 1 antibody
see all
Database links
- Entrez Gene: 466 Human
- Entrez Gene: 11908 Mouse
- Entrez Gene: 315305 Rat
- Omim: 123803 Human
- SwissProt: P18846 Human
- SwissProt: P81269 Mouse
- SwissProt: Q3KR79 Rat
- Unigene: 648565 Human
see all
Anti-ATF1 antibody images:
Western blot - ATF1 antibody (ab66016)
All lanes : Anti-ATF1 antibody (ab66016) at 1/500 dilution
Lane 1 : COLO205 cell extracts
Lane 2 : HeLa cell extracts
Lane 3 : HepG2 cell extracts
Lane 4 : HepG2 cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 29 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - ATF1 antibody (ab66016)
ab66016 at 1/500 dilution staining ATF1 in HUVEC cells by Immunofluorescence, in the absence or presence of the immunising peptide.
References for Anti-ATF1 antibody (ab66016)
ab66016 has not yet been referenced specifically in any publications.
Publishing research using ab66016? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"