You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-ATF1 (phospho S63) antibody [EP1591(2)Y]
See all ATF1 products (8) ...

Description

Rabbit monoclonal [EP1591(2)Y] to ATF1 (phospho S63)

Specificity

ab76085 detects ATF1 when phosphorylated at serine 63

Tested applications

WB, IHC-P, ICCmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF1

Positive control

WB: HeLa cell lysate IHC-P: human breast carcinoma

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1591(2)Y

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

  • Western blot - ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)Western blot - ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
WB: 1/50000 - 1/100000. Predicted molecular weight: 29 kDa.

Is unsuitable for Flow Cyt or IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.

Involvement in disease

Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.

Sequence similarities

Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP18846 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • TREB36 protein antibody
  • Activating transcription factor 1 antibody
  • ATF 1 antibody
  • atf1 antibody
  • ATF1 EWS fusion gene antibody
  • ATF1 FUS fusion gene antibody
  • ATF1_HUMAN antibody
  • cAMP dependent transcription factor 1 antibody
  • cAMP-dependent transcription factor ATF-1 antibody
  • Cyclic AMP dependent transcription factor ATF 1 antibody
  • Cyclic AMP dependent transcription factor ATF1 antibody
  • Cyclic AMP-dependent transcription factor ATF-1 antibody
  • EWS AFT1 antibody
  • FUS ATF 1 antibody
  • FUS/ATF 1 antibody
  • Protein TREB36 antibody
  • RNA binding protein activating transcription factor 1 fusion protein antibody
  • TREB 36 antibody
  • TREB36 antibody
see all

Database links

see all

Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] images:

  Western blot - ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)

Western blot - ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)

All lanes : Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085) at 1/100000 dilution

Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate, treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
goat anit rabbit HRP labelled IgG at 1/2000 dilution

Predicted band size : 29 kDa
Observed band size : 29 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)

Immunohistochemical analysis of ATF1 in paraffin embedded human breast carcinoma tissue, using ab76085 at a 1/100 dilution.

References for Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)

ab76085 has not yet been referenced specifically in any publications.

Publishing research using ab76085? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"