Overview

  • Product nameAnti-ATF2 antibody [E242]
    See all ATF2 primary antibodies
  • Description
    Rabbit monoclonal [E242] to ATF2
  • Specificityab32061 recognises ATF-2 (activating transcription factor-2, CRE-BP1). It does not cross-react with other ATF family members
  • Tested applicationsWB, ICC, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues N-terminus of human ATF-2

  • Positive control
    • HeLa cell lysate and human carcinoma
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab32061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
IHC-P
IP
  • Application notesICC: 1/250 - 1/500.
    IHC-P: 1/100.
    IP: 1/50.
    WB: 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).

    Is unsuitable for FACS.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
    • Tissue specificityAbundant expression seen in the brain.
    • Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP domain.
      Contains 1 C2H2-type zinc finger.
    • Post-translational
      modifications
      Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activating Transcription Factor 2 antibody
      • Activating Transcription Factor 2 antibody
      • Activating transcription factor 2 splice variant ATF2 var2 antibody
      • ATF 2 antibody
      • Atf-2 antibody
      • Atf2 antibody
      • ATF2 protein antibody
      • ATF2_HUMAN antibody
      • cAMP Response Element Binding Protein 2 antibody
      • cAMP Response Element Binding Protein 2 antibody
      • cAMP response element binding protein CRE BP1 antibody
      • cAMP response element-binding protein CRE-BP1 antibody
      • cAMP responsive element binding protein 2, formerly antibody
      • cAMP-dependent transcription factor ATF-2 antibody
      • cAMP-responsive element-binding protein 2 antibody
      • CRE BP1 antibody
      • CRE-BP antibody
      • CREB 2 antibody
      • CREB 2 antibody
      • CREB-2 antibody
      • CREB2 antibody
      • CREB2 antibody
      • CREBP1 antibody
      • CREBP1 antibody
      • Cyclic AMP dependent transcription factor ATF 2 antibody
      • Cyclic AMP-dependent transcription factor ATF-2 antibody
      • Cyclic AMP-responsive element-binding protein 2 antibody
      • D130078H02Rik antibody
      • D18875 antibody
      • HB 16 antibody
      • HB 16 antibody
      • HB16 antibody
      • HB16 antibody
      • Histone acetyltransferase ATF2 antibody
      • MGC105211 antibody
      • MGC105222 antibody
      • MGC111558 antibody
      • MGC142504 antibody
      • mXBP antibody
      • mXBP antibody
      • MXBP protein antibody
      • Tg(Gzma-Klra1)7Wum antibody
      • TREB 7 antibody
      • TREB7 antibody
      • TREB7 antibody
      see all

    Anti-ATF2 antibody [E242] images

    • Anti-ATF2 antibody [E242] (ab32061) at 1/10000 dilution + HeLa cell lysate

      Predicted band size : 54 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human carcinoma using ab32061 at a dilution of 1/50

    References for Anti-ATF2 antibody [E242] (ab32061)

    ab32061 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab32061.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"