Anti-ATF2 antibody [E243] (ab32160)

Overview

• Product nameAnti-ATF2 antibody [E243]See all ATF2 primary antibodies ...
• Description
  Rabbit monoclonal [E243] to ATF2
• SpecificityThis antibody recognises ATF2, but does not cross react with other ATF family members.
• Tested applicationsWB, IHC-P, ICC, IP more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A synthetic peptide corresponding to residues in C terminus of human ATF2.

• Positive controlWB: HeLa cell lysate. IHC-P: Breast carcinoma
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• Clonality Monoclonal
• Clone numberE243
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • Leucine Zipper

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
• Tissue specificityAbundant expression seen in the brain.
• Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
  Contains 1 bZIP domain.
  Contains 1 C2H2-type zinc finger.
• Post-translational
  modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
• Cellular localizationNucleus.

Target information above from: UniProt accession P15336 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1386 Human
  • Omim: 123811 Human
  • SwissProt: P15336 Human
  • Unigene: 592510 Human

• Alternative names
  Activating Transcription Factor 2 antibodyActivating transcription factor 2 splice variant ATF2 var2 antibodyATF 2 antibody

  Atf-2 antibodyAtf2 antibodyATF2 protein antibodyATF2_HUMAN antibodycAMP Response Element Binding Protein 2 antibodycAMP response element binding protein CRE BP1 antibodycAMP response element-binding protein CRE-BP1 antibodycAMP responsive element binding protein 2, formerly antibodycAMP-dependent transcription factor ATF-2 antibodycAMP-responsive element-binding protein 2 antibodyCRE BP1 antibodyCRE-BP antibodyCREB 2 antibodyCREB 2 antibodyCREB-2 antibodyCREB2 antibodyCREBP1 antibodyCyclic AMP dependent transcription factor ATF 2 antibodyCyclic AMP-dependent transcription factor ATF-2 antibodyCyclic AMP-responsive element-binding protein 2 antibodyD130078H02Rik antibodyD18875 antibodyHB 16 antibodyHB 16 antibodyHB16 antibodyMGC105211 antibodyMGC105222 antibodyMGC111558 antibodyMGC142504 antibodymXBP antibodyMXBP protein antibodyTg(Gzma-Klra1)7Wum antibodyTREB 7 antibodyTREB7 antibody

  see all

Anti-ATF2 antibody [E243] images

• 

  Western blot - ATF2 antibody [E243] (ab32160)
  Anti-ATF2 antibody [E243] (ab32160) at 1/10000 dilution + HeLa cell lysate
  
  Predicted band size : 54 kDa
  Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody [E243] (ab32160)
  Ab32160, at a dilution of 1/250, staining ATF2 in paraffin embedded breast carcinoma tissue by Immunohistochemistry.