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Anti-ATF2 antibody [E243] (ab32160)

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Overview

Product name

Anti-ATF2 antibody [E243]
See all ATF2 products (32) ...

Description

Rabbit monoclonal [E243] to ATF2

Specificity

This antibody recognises ATF2, but does not cross react with other ATF family members.

Tested applications

WB, IHC-P, ICC, IPmore details

Cross reactivity

Reacts with

Mouse, Human

Does not react with

Rat

Immunogen

A synthetic peptide corresponding to residues in C terminus of human ATF2.

Positive control

WB: HeLa cell lysate. IHC-P: Breast carcinoma

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

E243

Isotype

IgG

Research areas

Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

  • Western blot - ATF2 antibody [E243] (ab32160)Western blot - ATF2 antibody [E243] (ab32160) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody [E243] (ab32160)Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody [E243] (ab32160) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: 1/250 - 1/500.
IHC-P: 1/250 - 1/500.
IP: 1/60.
WB: 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).

Is unsuitable for FACS.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.

Tissue specificity

Abundant expression seen in the brain.

Sequence similarities

Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.

Post-translational
modifications

Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP15336 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CREB 2 antibody
  • HB 16 antibody
  • Activating Transcription Factor 2 antibody
  • ATF 2 antibody
  • Atf-2 antibody
  • Atf2 antibody
  • ATF2 protein antibody
  • ATF2_HUMAN antibody
  • cAMP Response Element Binding Protein 2 antibody
  • cAMP response element binding protein CRE BP1 antibody
  • cAMP response element-binding protein CRE-BP1 antibody
  • cAMP-dependent transcription factor ATF-2 antibody
  • cAMP-responsive element-binding protein 2 antibody
  • CRE BP1 antibody
  • CRE-BP antibody
  • CREB 2 antibody
  • CREB-2 antibody
  • CREB2 antibody
  • CREBP1 antibody
  • Cyclic AMP dependent transcription factor ATF 2 antibody
  • Cyclic AMP-dependent transcription factor ATF-2 antibody
  • Cyclic AMP-responsive element-binding protein 2 antibody
  • D130078H02Rik antibody
  • D18875 antibody
  • HB 16 antibody
  • HB16 antibody
  • MGC105211 antibody
  • MGC105222 antibody
  • MGC111558 antibody
  • MGC142504 antibody
  • mXBP antibody
  • MXBP protein antibody
  • Tg(Gzma-Klra1)7Wum antibody
  • TREB 7 antibody
  • TREB7 antibody
see all

Database links

Anti-ATF2 antibody [E243] images:

  Western blot - ATF2 antibody [E243] (ab32160)

Western blot - ATF2 antibody [E243] (ab32160)

Anti-ATF2 antibody [E243] (ab32160) at 1/10000 dilution + HeLa cell lysate

Predicted band size : 54 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody [E243] (ab32160)

Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody [E243] (ab32160)

Ab32160, at a dilution of 1/250, staining ATF2 in paraffin embedded breast carcinoma tissue by Immunohistochemistry.

References for Anti-ATF2 antibody [E243] (ab32160)

ab32160 has not yet been referenced specifically in any publications.

Publishing research using ab32160? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"