ATF2 antibody [E243] (ab32160)

Product Name 

ATF2 antibody [E243]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [E243] to ATF2

Immunogen 

A synthetic peptide corresponding to residues in C terminus of human ATF2.

Reacts with 

Hu, Ms

Does not react with 
(species key)

Rat

Specificity 

This antibody recognises ATF2, but does not cross react with other ATF family members.

Tested applications 
(see key)

ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/250 - 1/500.
IHC-P: 1/250 - 1/500.
IP: 1/60.
WB: 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).

Is unsuitable for FACS.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: HeLa cell lysate.
IHC-P: Breast carcinoma

Cellular localization 

Nuclear

Research areas 

Immunology >> Innate Immunity >> TLR Signaling
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper

Relevance 

ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

E243

Isotype 

IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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