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Product name
Anti-ATF2 antibody
See all ATF2 products (33) ...
Description
Rabbit polyclonal to ATF2
Specificity
This antibody detects endogenous levels of ATF2 protein around Threonine 73 or 55.
Tested applications
WB, ELISA, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide around the phosphorylation site of Threonine 73 or 55 (Human)
Positive control
IHC-P: Breast Carcinoma
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide.
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IP: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100.
Target
Function
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificity
Abundant expression seen in the brain.
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
Post-translational
modifications
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP15336
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CREB 2 antibody
- HB 16 antibody
- Activating Transcription Factor 2 antibody
see all
Database links
- Entrez Gene: 1386 Human
- Entrez Gene: 100047997 Mouse
- Entrez Gene: 11909 Mouse
- Entrez Gene: 81647 Rat
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: P16951 Mouse
- SwissProt: Q00969 Rat
see all
Anti-ATF2 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - ATF2 antibody (ab31483)
Ab31483, at a dilution of 1/50, staining ATF2 in paraffin embedded breast carcinoma tissue byImmunohistochemistry.
Left image: Using ab31483.
Right image: The same antibody preincubated with synthesized peptide.
Western blot - ATF2 antibody (ab31483)
All lanes : Anti-ATF2 antibody (ab31483) at 1/500 dilution
Lane 1 : Extracts from LOVO cells (5-30ug) treated with ab31483.
Lane 2 : Extracts from LOVO cells (5-30ug) treated using the same antibody preincubated with
synthesized peptide.
Secondary
goat-anti-rabbit IgG-AP-conjugate.
Predicted band size : 54 kDa
References for Anti-ATF2 antibody (ab31483)
ab31483 has not yet been referenced specifically in any publications.
Publishing research using ab31483? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"